Ordered Coimmobilization of Multimeric Enzyme Arrays with Enhanced Biocatalytic Cascade Performance.
ACS applied bio materials(2021)
摘要
Multiple enzyme coimmobilizations mimicking nature cascade enzymatic pathways have potential applications in diverse fields. We have developed a strategy for orderly coimmobilizing multienzymes by combining hierarchically self-assembled multimeric enzymes with specifically abundant polyhistidine tag affinity-mediated immobilization. Using this strategy, an ordered coimmobilization of the glycosyltransferase UGT51 mutant and sucrose synthase was constructed to realize the regeneration of costly sugar donor UDP-glucose that was used in the biosynthesis of the rare ginsenoside Rh2. The ordered coimmobilization array not only significantly boosted the immobilization and catalysis efficiency but also improved UDP-glucose regeneration, storage stability, and reusability compared to those of random coimmobilization and free enzyme-assembly systems. This study provides a great promise for fabricating enzyme arrays and highlights the synergistic benefits of nanocomplexes in enhancing biocatalytic cascade performance.
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关键词
multimeric enzymes,self-assembly,coimmobilizations,glycosyltransferase,sucrose synthase,UDP-glucose regeneration
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