Supplementary Data File from Autofluorescence Imaging of 3D Tumor–Macrophage Microscale Cultures Resolves Spatial and Temporal Dynamics of Macrophage Metabolism

Supplementary figures 1-5 and supplementary tables 1-3. Supplementary Figure 1: Fluorescence lifetimes of NAD(P)H and FAD exhibit differences between macrophage polarization states. Supplementary Figure 2: Validation of two-photon wavelength mixing for evaluating autofluorescence and migration effects in 2D cultures and the 3D Stacks microfluidic platform. Supplementary Figure 3: Prolonged co-culture of mouse breast cancer and macrophages yields heterogeneous NAD(P)H and FAD fluorescence lifetime and migration compared to monoculture. Supplementary Figure 4: Primary human tumor cells stimulate changes in NAD(P)H and FAD fluorescence lifetime and cell migration in co-cultured human monocyte-derived macrophages. Supplementary Figure 5: Metabolic changes in human THP-1s following co-culture with MDA-MB-231 human breast carcinoma. Supplementary Table 1: Mouse and Human Macrophage Polarization Gene Panels. Supplementary Table 2: Significance of redox ratio fold change in inhibitor-treated 2D cytokine-stimulated mouse macrophages. Supplementary Table 3: Coefficients of variation (CV) and significance of CV equality for optical redox ratio in 2D cytokine-stimulated mouse macrophages and 3D mouse monocultures and co-cultures.