"开展畜禽品种选育,畜禽营养饲料、动物生物技术及畜禽疫病防治等研究;开展相关的技术咨询、服务及产品开发。 "
The newly collected and viability semen of lake sheep was added to the frozen basal dilu-tion containing nanoparticulate selenium(SeNPs)with different mass concentrations(0.00,0.25,0.50,1.00,2.00 mg/L,groups Ⅰ-Ⅴ)for dilution,and then the diluted semen was divided into 0.25 mL frozen fine tubes and stored in liquid nitrogen.After 7 d of thawing,sperm quality param-eters were evaluated for each treated semen,including sperm motility,motility parameters,malfor-mation rate,acrosome integrity rate,plasma membrane integrity,and the total antioxidant capacity(T-AOC),catalase activity(CAT),reactive oxygen species(ROS)and malondialdehyde(MDA)content of each group of semen and the expression of related antioxidant genes(SOD1,GCLC,GCLM and PRDX1)were determined.The results showed that compared with group Ⅰ,groups Ⅲand Ⅳ had positive effects on sperm motility,acrosome integrity rate and membrane integrity after thawing(P＜0.05),especially the post-freezing motility of sperm in group Ⅳ reached 48.68％,the acrosome integrity rate was 80.51％,the plasma membrane integrity rate of the head was 52.54％,and the integrity rate of the caudal plasma membrane was 50.99％,all at the highest level.The T-AOC and CAT activities of frozen semen treated with SeNPs increased significantly(P＜0.05),and the highest T-AOC in group Ⅳ was(10.94±0.25)U/mL,and CAT activity was(11.01±0.92)U/mL.SeNPs significantly reduced MDA and ROS levels in spermatozoa after defrost and were best treated at a mass concentration of 1.0 mg/L(P＜0.05),MDA content could be reduced to(4.81±0.36)mmol/L,and ROS levels were 1 880.98±44.86.The antioxidant gene assay showed that SOD1 was extremely significantly higher in the 1.00 mg/L SeNPs-treated group than in the 0.00 mg/L SeNPs-treated group(P＜0.01),and GCLM,GCLC and PRDX1 were significantly higher than in the 0.00 mg/L SeNPs-treated group(P＜0.05).The results indicated that the SeNPs mass concentration of 1.00 mg/L within the frozen dilution solution of lake sheep resulted in an overall improvement in sperm structure and function,and also had a positive effect on the an-tioxidant capacity of frozen-thawed sperm.
