贵州医科大学

Chronic pain profoundly undermines patients' physical,psychological,and social functioning,highlighting the urgent need for effective coping and management strategies.Psychological resilience plays a pivotal role in mitigating the adverse impact of pain,and enhancing resilience has become essential for patients to face biopsychosocial challenges.However,the key factors that promote pain resilience and their underlying mechanisms remain unclear.Previous studies indicate a positive association between cognitive function and resilience,with interventions targeting cognitive interventions shown to strengthen resilience.Building on this work,studies employing neuroimaging and machine learning techniques further revealed that gray matter volume and functional activity within cortical regions of the central executive network(CEN)are associated with pain resilience.Building on these findings,we hypothesize that cognitive function and the central executive network are not only pivotal for enhancing pain resilience but also predictive of its development.To test this,we will conduct a multicenter,longitudinal study combining self-report assessments and functional MRI data.Specifically,we will:1)apply cross-lagged panel analysis to uncover temporal associations between cognitive function and pain resilience;2)examine the mediating role of resting-state functional connectivity within the central executive network in the relationship between cognitive function and pain resilience;and 3)employ a Gated Recurrent Unit(GRU)-based deep learning algorithm for temporal data modeling to construct and validate a multimodal predictive model of pain resilience.This study offers a novel neuroimaging perspective on chronic pain coping and provides a scientific foundation for developing more effective pain management and precision treatment strategies.

To investigate the intervention effects of Ligustrum robustum extract(SLKDTE)on hyperlipidemia,a strategy combining transcriptomic analysis and experimental validation was employed.By administering the extract to mice concurrently with an 8-week high-fat diet,the pharmacological intervention effects and underlying mechanisms were objectively evaluated.The results showed that SLKDTE could significantly inhibit weight gain caused by a high-fat diet.The content of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),glucose(Glu),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)in serum was all significantly decreased,and the level of high-density lipoprotein cholesterol(HDL-C)increased significantly.Moreover,SLKDTE reduced the serum levels of tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),and lipopolysaccharide(LPS)in mice.The findings indicated that it had the effects of regulating serum lipid levels and inhibiting inflammation.The histopathological results showed that the accumulation of lipid droplets in the liver decreased,and the levels of ALT,AST,TC,and TG in the liver decreased significantly,suggesting that SLKDTE had the alleviating effect on hepatic lipid deposition.The results of transcriptome sequencing and Western blot verification showed that it significantly upregulated the expression of medium-chain acyl-CoA dehydrogenase(ACADM),hydroxyacyl-CoA dehydrogenase β subunit(HADHB),and enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase(EHHADH)proteins,promoting β-oxidation of fatty acids.This study preliminarily revealed that SLKDTE could reduce the risk of hyperlipidemia in mice by interfering with key enzymes in the fatty acid degradation pathway.It provides preliminary experimental basis and clues for the subsequent in-depth development and utilization of L.robustum.

Background Post-tuberculosis bronchiectasis is one of the significant sequelae of tuberculosis.At present,the specific impact on the prognosis of patients with tuberculous bronchiectasis has not been fully understood.Objective To understand the prognosis and influencing factors of patients with post-tuberculosis bronchiectasis and provide scientific basis for improving the prognosis of these patients.Methods A total of 337 patients with post-tuberculosis bronchiectasis who were hospitalized in Guiyang Public Health Treatment Center from April 2023 to April 2024 were selected.Telephone follow-up was conducted on their prognosis 6 months after discharge,and they were divided into the good prognosis group and the poor prognosis group.The random forest model and LASSO regression were used to screen and analyze the influencing factors of the prognosis and rank their importance.Results The incidence of poor prognosis in 337 patients with post-tuberculosis bronchiectasis was 41.2%(139/337).There were statistically significant differences in age,educational level,BMI,previous smoking,the percentage of forced expiratory volume in one second(FEV1%pred),alveolar lavage,combined chronic pulmonary heart disease,combined chronic obstructive pulmonary disease,combined hypertension,Pseudomonas aeruginosa infection,post-discharge smoking,post-discharge drinking,lack of daily exercise,living alone,non-compliance with medication as prescribed,and levels of interleukin-6(IL-6)and high-sensitivity C-reactive protein(hs-CRP)at discharge between the two groups(P<0.05).LASSO regression model analysis was conducted on the variables with significant differences in the univariate analysis,and a total of 9 variables were selected.The results of the random forest model showed that the order of importance of each influencing factor was as follows:non-compliance with medication as prescribed,post-discharge smoking,lack of daily exercise,educational level,combined chronic pulmonary heart disease,discharge IL-6,age,post-discharge drinking,and living alone.The results of the multivariate Logistic regression analysis showed that non-compliance with medication as prescribed(OR=8.394,95%CI=3.323-21.203),post-discharge smoking(OR=5.294,95%CI=2.313-12.116),lack of daily exercise(OR=4.555,95%CI=2.206-9.406),combined chronic pulmonary heart disease(OR=4.331,95%CI=2.012-9.323),discharge IL-6(OR=1.054,95%CI=1.018-1.091),and age≥80 years(OR=5.246,95%CI=1.097-25.077)were risk factors for the prognosis of patients with post-tuberculosis bronchiectasis(P<0.05),while primary education(OR=0.358,95%CI=0.143-0.898)and secondary education(OR=0.202,95%CI=0.074-0.554)were protective factors for the prognosis of patients with post-tuberculosis bronchiectasis(P<0.05).Conclusion Patients with post-tuberculosis bronchiectasis have a relatively high incidence of poor prognosis.Non-compliance with medication as prescribed,smoking after discharge,lack of daily exercise,combined chronic obstructive pulmonary disease,IL-6 at discharge,age≥80 years old,and educational level are important influencing factors for tuberculous bronchiectasis.Targeted measures can be taken based on these influencing factors.