一般项目：第一类医疗器械生产(限分支机构经营）；第二类医疗器械销售；第一类医疗器械销售；工程和技术研究和试验发展；医学研究和试验发展；技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广；医疗设备租赁；非居住房地产租赁；货物进出口；技术进出口。（除依法须经批准的项目外，凭营业执照依法自主开展经营活动）许可项目：第三类医疗器械生产；第二类医疗器械生产；第三类医疗器械经营；医疗器械互联网信息服务。（依法须经批准的项目，经相关部门批准后方可开展经营活动，具体经营项目以相关部门批准文件或许可证件为准）
Objective To discuss application value of hypervariable regions (V3,V6) of respiratory pathogens 16S ribosomal RNA (rRNA) gene in liquid chip system.Methods According to respiratory pathogens 16S rRNA gene sequences from GenBank,the primers and the probes were designed and liquid chip system was established.52 DNA samples from sputum of patients with respiratory infections in Fujian provincial hospital were detected by liquid chip system.Comparing with sequencing technology,the sensitivity and specificity were analyzed.Results There were differences in hypervariable regions (V3,V6) of respiratory pathogens 16S rRNA genes.It took only 3.5 hours for liquid chip system detection.Probe of hypervariable regions V6 of Psudomonas aeruginosa 16S rRNA gene could detect ＞ 102/μl DNA samples,threshold,the sensitivity was 100.0％ and specificity was 100.0％.The sensitivity of probe of hypervariable regions V3 of Staphylococcus aureus 16S rRNA gene was 66.7％,and specificity was 98.0％.The sensitivity of probe of hypervariable regions V6 of Staphylococcus aureus 16S rRNA gene was 0.0％.Conclusions The differences in hypervariable regions(V3,V6) of respiratory pathogens 16S rRNA genes can be applied to etiology diagnosis,but not for all respiratory pathogens.
Objective To explore a new multiplex methylation specific polymerase chain reaction(PCR) method for analyzing the methylation status of 10 lung cancer genes.Methods A total of 19 hospitalized patients with lung cancer were selected,including 16 males and 3 females with mean age of 57.3 years old.A 52-year-old male patient with pulmonary benign lesion was also enrolled in the present study.The methylation and unmethylaiton standards of 10 lung cancer-related genes were set up by treating the plasmids encoding the genes with DNA methyltransferase and sulfite.Ten pairs of methylation specific primers were selected for the multiplex-methylation specific PCR and the condition for the PCR were optimized by choosing the best optimal polymerase and annealing temperature.The methylation rate of 10 lung cancer-related genes from the patients was detected using the multiplex methylation specific PCR.Results The methylation and unmethylaiton standards of 10 lung cancer genes were set up successfully and the muliplex-methylation specific PCR were able to test the methylation status of the 10 genes simultaneously with perfect consistency.The methylation rate is 92 %,89 %,76 %,63 %,50 %,39 %,21 %,11 %,and 0 % for p16INK4A,H-cadherin,E-cadherin and DAPK,TIMP-3,RARβ,MGMT,RASSF1A,GSTP1 and hMLH1,respectively.Conclusion It is demonstrated that the new multiplex methylation specific PCR could be utilized to evaluate the methylation status of 10 lung cancer-related genes simultaneously and have the potential in clinical diagnosis.
