一般项目：技术服务、技术开发、技术咨询、技术交流、技术转让、技术推广；畜牧机械制造；畜牧机械销售；实验分析仪器制造；玻璃仪器制造；仪器仪表制造；实验分析仪器销售；仪器仪表销售；其他通用仪器制造；企业管理；企业管理咨询；财务咨询；票据信息咨询服务；信息咨询服务（不含许可类信息咨询服务）；教育咨询服务（不含涉许可审批的教育培训活动）；医学研究和试验发展；专用化学产品销售（不含危险化学品）；生物基材料技术研发；工程和技术研究和试验发展；生物基材料销售；工业酶制剂研发；海洋生物活性物质提取、纯化、合成技术研发；细胞技术研发和应用；发酵过程优化技术研发；第一类医疗器械生产；第一类医疗器械销售；第二类医疗器械销售；物联网技术服务（除依法须经批准的项目外，凭营业执照依法自主开展经营活动）许可项目：第二类医疗器械生产；第三类医疗器械生产；第三类医疗器械经营；兽药生产；兽药经营；检验检测服务；用于传染病防治的消毒产品生产；药品生产；药品进出口；技术进出口；货物进出口；牲畜饲养；种畜禽生产；出版物零售（依法须经批准的项目，经相关部门批准后方可开展经营活动，具体经营项目以审批结果为准）
The experiment aimed to explore the effects of a Bacillus subtilis compound preparation on the production performance and intestinal microbiota of laying hens.Thirty 500-day-old hens with similar body weights were randomly divided into two groups,with three replicates per group,and each replicate consisted of five hens.The CK group(control group)was fed a corn-soybean meal basic diet,while the T group was supplemented with 1 g/kg Bacillus subtilis compound preparation in the basic diet.The trial lasted for 70 days.The results showed that compared with the CK group,the feed-to-egg ratio in the T group was significantly reduced(P＜0.05).At the phylum level,the relative abundance of the Firmicutes in the duodenum was extremely increased(P＜0.01),the relative abundance of the Actinobacteria in the ileum was significantly increased(P＜0.05),and the relative abundance of the Proteobacteria in the cecum was extremely decreased(P＜0.01).At the genus level,the relative abundance of Lactobacillus in the duodenum was extremely increased(P＜0.01),the abundance of Corynebacterium was extremely decreased(P＜0.01),and the relative abundance of Enterococcus and Aeriscardovia in the ileum was significantly or extremely significantly increased(P＜0.05 or P＜0.01),and the relative abundance of the Ruminococcaceae in the cecum was significantly increased(P＜0.05).β-diversity analysis showed that the microbial community structures of the duodenum and ileum had certain similarities and were different from the cecal microbial community structure.The study suggests that the addition of Bacillus subtilis compound preparation can promote the growth of lactic acid bacteria and Aeriscardovia,playing a role in optimizing the intestinal microbiota and improving feed utilization.
The objective of this study was to establish a real-time PCR method for detection of the proliferation of goose parvovirus（GPV）in goose embryo epithelial cells（GEEC）.The fluorescence dye SYBR GreenⅠinfiltration method was used and the growth of GPV in GEEC was analyzed and the growth curve was drawn.The results showed that the standard curve established by SYBR GreenⅠquantitative real-time PCR assay showed good repeatability and specificity,and it showed a good linear relationship with template concentration.In the linear concentration range,with the decrease of the template amount,the corresponding Ct value increases correspondingly,and the standard curve equation is y=-3.341 2x+38.494,R 2 is 0.998.Sensitivity test showed that the lowest detection limit was 100copies/reaction,while the lowest detection limit of common PCR was 1000copies/reaction.The melting curve is a single characteristic peak,and the melting temperature is 80℃+0.5℃．The virus growth curve showed that the virus had a good proliferation in GEEC,and the amount of 60hincreased gradually,indicating that it was rapidly proliferating before 72h,and the amount of virus reached the highest in 72h.This result is consistent with that of synchronous TCID 50 assay,and it is more sensitive and accurate.It provides a rapid and effective method for the quantitative detection of goose parvovirus.
