国有资产经营管理；基础设施建设、房地产开发经营、园林绿化工程施工；物业管理；土石方工程服务；农业种植、水产养殖；非金属矿开采、围垦造地工程石料供应、建筑材料、矿产品（不含煤炭）的销售、砂石料经营管理；普通货物仓储（不含危险化学品和食品）。
NAC transcription factors are widely present in plants and unique to plants, which play important roles in regulating plant growth and development, hormone signaling, and the response to adverse stress. In our previous work, a low-temperature-induced NAC gene, KoNAC25(GenBank accession number PP860407), was identified from the genome of the mangrove Kandelia obovata. To investigate whether the KoNAC25 gene of K. obovata is involved in the response to salt stress conditions, this study cloned the KoNAC25 gene from the cDNA of K. obovata leaves via RT-PCR and performed sequence characterization, phylogenetic evolution, subcellular localization, and gene expression analyses. The results were as follows:(1)The open reading frame(ORF)of the KoNAC25 gene was 858 bp in length and encoded 285 amino acids, with a molecular weight of 32.9 kDa and a theoretical isoelectric point of 8.53. KoNAC25 was a hydrophilic protein and contained no signal peptide or transmembrane structure, and its secondary structure was predominantly random coils(58.95%).(2)Amino acid sequence homology alignment showed the N-terminus of the KoNAC25 protein had a NAM superfamily structural domain, which consisted of five conserved substructural domains(ABCDE)and belonged to the NAM subfamily.(3)The phylogenetic tree revealed that the KoNAC25 transcription factor of K. obovata was similar to the transcription factors of Ricinus communis, Manihot esculenta, and Hevea brasiliensis.(4)The subcellular localization test confirmed that the protein was localized in the nucleus, which was consistent with bioinformatics prediction.(5)qRT-PCR analysis result revealed that the expression of the KoNAC25 gene was significantly greater in leaves than in flowers and could be induced to upregulate its expression by NaCl(200 mmol·L-1)and abscisic acid(ABA)(100 μmol·L-1), which peaked under 12 h treatment and 6 h treatment, respectively; however, the expression level of this gene was not induced by salicylic acid(100 μmol·L-1)and methyl jasmonate(100 μmol·L-1). In summary, KoNAC25 is involved in the related regulation of the salt stress response in Kandelia obovata, which provides a reference for further research on the function of KoNAC25 gene and its expression regulatory mechanism under salt stress in K. obovata.
