多肽及蛋白质类医药中间体（不含危险品）、其他生物化学试剂（不含危险品）、涂层材料、消毒产品、化妆品、其他日用化学品的研究、开发、销售；生物医药技术的研究、开发、转让、推广、应用、咨询；化工产品（不含危险品）、纳米结构材料（不含化学原料及制品、不含危险品）、包装材料（不含危险品）、其他生物材料（不含危险品）、无机盐（不含食盐、不含危险品）、医疗器械（不含许可经营项目）的销售；二类6864医用卫生材料及敷料、三类6864医用卫生材料及敷料、三类6865医用缝合材料及粘合剂的生产；自营和代理各类商品及技术的进出口业务，但国家限定企业经营或禁止进出口的商品和技术除外。（依法须经批准的项目，经相关部门批准后方可开展经营活动）
OBJECTIVE:To investigate the feasibility of using liquid chromatography (HPLC) to characterize the 3, 4-Dihydroxyphenylalanine (DOPA) redox state of mussel adhesive protein (MAP).METHODS:The DOPA and protein contents of MAP were determined by HPLC, Arnow and Bradford methods respectively.RESULTS:With extended oxidation time, the protein contents of MAP samples remained unchanged whereas the DOPA contents declined. The retention times of main peaks in HPLC for both the accelerated oxidation and retained samples shifted as the storage time extended, which could be related to the changes of sample redox state.CONCLUSIONS:The redox state of MAP can be characterized by the change of HPLC peak retention time. HPLC can be used in the research on the MAP redox state, which is beneficial to the product development and quality control.
