口腔内科专业,口腔外科专业,口腔修复科专业,口腔正畸专业,口腔预防保健专业。
Objective To investigate the distribution and antimicrobial susceptibility of anaerobic strains isolated from gingival crevicular fluid of patients with periodontitis. Methods Gingival crevicular fluid was collected from 40 adults with periodontitis in Huashan Hospital, Fudan University, Jing’an District Dental Institute, and Jiading District Dental Institute from December 2020 to May 2021. Anaerobic bacteria were cultured with the specimens and identified by matrix-assisted laser desorption/ionization timeof-flight mass spectrometry（MALDI-TOF MS）. The susceptibility of the isolates to 12 antimicrobial agents was determined by agar dilution method. Results A total of 192 anaerobic bacteria were isolated. The top five species were Prevotella intermedia（65.0%）,Prevotella nigrescens（55.0%）, Fusobacterium nucleatum（52.5%）, Capnocytophaga ochracea（37.5%）, and Actinomyces viscosus（30.0%）. Overall, 96 isolates were successfully revived, all of which were susceptible to amoxicillin-clavulanic acid and meropenem.Almost all isolates except Prevotella spp.（61.3% susceptible） were susceptible to amoxicillin. Prevotella spp. and Veillonella spp. were all susceptible to metronidazole, while Actinomyces spp. and Granulicatella spp. were nearly 100% resistant to metronidazole. All other isolates except Veillonella spp. were susceptible to doxycycline and moxifloxacin. Conclusions Prevotella spp., Fusobacterium spp. and Capnocytophaga spp. were the prevalent anaerobic bacteria in the gingival crevicular fluid of periodontitis patients. Most of the isolates were susceptible to amoxicillin, amoxicillinclavulanic acid, doxycycline, and metronidazole.
