Background Marfan syndrome (MS) is a rare, life-threatening connective tissue disorder. Short video platforms are now key health-information sources, yet the quality and reliability of MS-related content remain unclear. Methods In a cross-sectional study, Marfan syndrome videos were retrieved from Bilibili and Douyin on 25 April 2026 using the Chinese keyword “马凡综合征,” from new accounts under anonymous browsing. After screening, data on video characteristics, uploader type, and publicly displayed likes, comments, shares, and collections were extracted. Two reviewers independently scored all eligible videos with Global Quality Scale (GQS), modified Decision-making Information Support Criteria for Evaluating the Reliability of Non-randomised Studies (mDISCERN), Journal of the American Medical Association (JAMA) criteria, and Content Completeness Score (CCS). Descriptive statistics, non-parametric tests, Spearman correlations, and weighted Cohen’s kappa were used. Results 185 videos (103 from Douyin and 82 from Bilibili) were analyzed. The median video duration was significantly longer on Bilibili than on Douyin (126.00 (IQR: 59.00–377.00) vs. 64.00 (IQR: 45.50–103.50) seconds; P < 0.001). Douyin videos had been available for a significantly shorter time since publication (median: 275.00 vs. 747.50 days; P < 0.001), yet their per-day interaction counts remained significantly higher after this adjustment (all P < 0.001). Douyin videos demonstrated significantly higher absolute interaction counts, including likes (median: 2,214.00 vs. 48.00; P < 0.001), comments (240.00 vs. 0.00; P < 0.001), shares (606.00 vs. 5.50; P < 0.001), and collections (475.00 vs. 24.00; P < 0.001). In terms of quality, Douyin videos had higher GQS scores than Bilibili (median: 4.00 vs. 3.00; P < 0.001), whereas Bilibili videos showed higher mDISCERN scores (median: 3.00 vs. 2.00; P < 0.001). No significant differences were observed in JAMA scores ( P = 0.245) or CCS ( P = 0.274). Healthcare professionals accounted for the majority of Douyin content (78.6%), while institutional sources predominated on Bilibili (53.7%) ( P < 0.001). Videos produced by professionals and institutions achieved significantly higher quality and reliability scores compared to individual uploaders. Spearman correlation analysis revealed strong correlations among engagement metrics ( r = 0.84–0.94), but weak or negligible correlations between absolute interaction counts and quality scores ( r = −0.16 to 0.17). Conclusion Marfan syndrome-related videos on Bilibili and Douyin varied considerably in educational quality and reliability; those by healthcare professionals were generally higher quality. Within this sample, observed interaction counts showed weak or negligible associations with quality measures.
Colorectal cancer (CRC) is a significant global health concern, with adenomas representing critical precancerous lesions. Early detection of adenomas is essential for reducing the incidence of CRC; however, effective biomarkers for the adenoma–carcinoma transition are currently lacking. Serine tRNA ligase (SARS1) has been identified in colorectal adenoma tissues, but its biological role remains unclear. Here, we analyzed SARS1 expression in colorectal adenoma and colorectal cancer using data from the GEO and TCGA databases, as well as immunohistochemical staining of 48 tissue pairs (normal colorectal mucosa, adenoma lesion, and cancer lesion). SARS1 expression was significantly higher in adenomas and cancers compared to normal colorectal mucosa, decreasing from the adenoma to cancer stages. High SARS1 expression was associated with improved overall survival (OS; Log-rank P = 0.0209) and progression-free survival (PFS; Log-rank P = 0.0147) in patients with CRC. As demonstrated by immunofluorescence and qRT-PCR, in colorectal adenoma organoids, SARS1 knockdown was associated with decreased expression of LGR5 and OLFM4, and increased expression of c-Myc. This gene expression profile mirrors that observed in colorectal cancer organoids, as reported in previous studies. Additionally, the downregulation of SARS1 also enhanced the proliferation of CRC cells and the viability of colorectal adenoma organoids ( p < 0.01 to p < 0.0001). Our findings suggest that SARS1 exerts context-dependent, stage-associated growth-suppressive effects during the adenoma-to-carcinoma transition, and its downregulation is associated with colorectal cancer progression. SARS1 represents a candidate marker reflecting adenoma-to-carcinoma progression; however, its clinical utility as a diagnostic or prognostic tool requires independent validation in larger, independent, adequately powered cohorts, and its precise functional mechanisms warrant further investigation.
Soil salinization severely restricts plant growth and threatens ecological sustainability, particularly in soda saline-alkali regions such as the Songnen Plain. Asteraceae plants are widely used in vegetation restoration due to their strong ecological adaptability. In this study, Tagetes erecta L., Cadendula officinalis L., and Leucanthemum maximum (Ramood) DC were selected as experimental materials. Two types of stress (salt and alkali) were applied at five concentration levels each (excluding the distilled-water control), which respectively constituted a salinity gradient and an alkalinity gradient. Seed germination parameters, seedling growth traits, and physiological indicators were measured, and field validation was conducted in areas with mild, moderate, and severe saline-alkali soils in the Songnen Plain. T. erecta L. showed optimal germination at the low concentration of salt and alkalinity (25 mmol/L), exhibiting a “low promotion and high inhibition” pattern, and maintained relatively high vigor under high stress. L. maximum had the highest germination rate under non stress conditions but was highly sensitive to increasing salt and alkalinity, while its root growth remained relatively stable under salt stress. C. officinalis L. was highly sensitive to alkali stress. Alkali stress consistently exerted stronger inhibitory effects on seed germination and seedling growth than salt stress. Field validation further demonstrated that T. erecta L. maintained a significantly higher survival rate under severe saline-alkali conditions (HS) compared to L. maximum and C. officinalis L. ( p < 0.05). This study identified interspecific differences in saline-alkali tolerance among three Asteraceae species, offering a data basis and scientific reference for plant selection and breeding in ecological restoration and landscaping of saline-alkali areas.
Yeast Saccharomyces cerevisiae is a widespread model system in which mechanisms of prion induction and elimination have been identified. New and safe substances and methods are being sought to eliminate prions from cells. In this research, we applied guanidinium hydrochloride (GuHCl) alone and in combination with pulsed electric field treatment against [ PSI + ] prion. The analysis of cell culture optical density and doubling time showed that the method of [ PSI + ] prion elimination increases cell doubling time, with combined treatment having a more severe impact on cell growth than GuHCl treatment alone. Additionally, we found that both elimination methods can impact the optical culture density achieved by [ psi − ] cells. However, the cells in which the [ PSI + ] prion has been eliminated by GuHCl alone are able to reach the same optical density as unaffected [ psi − ] cells and a higher optical density than the affected [ psi − ] cells by GuHCl alone. These findings suggest that the presence and subsequent removal of the [ PSI + ] prion influence early exponential growth, revealing lasting effects on cell growth dynamics that are distinct from previously reported effects on stationary phase survival. This work highlights how prion elimination strategies, including chemical and electric field treatments, can impact post-curing growth behavior in yeast cells.
Biting behavior by males during mating has been widely documented in elasmobranchs; however, its characteristics in the whale shark, Rhincodon typus , remain poorly understood. In this study, we recorded a pectoral fin biting event by a male directed toward a female at Okinawa Churaumi Aquarium on May 12, 2020, using surveillance cameras. Subsequently, we monitored the healing process of the resulting scars through underwater observations and post-mortem tissue analyses over 13 months. The bite marks initially appeared as open wounds, which closed within approximately 4 weeks, and were followed by the regeneration of dermal denticles and progressive melanization that persisted for more than 13 months. Additionally, 523 bite marks (233 dorsal and 290 ventral) were digitized and analyzed using orientation-based analysis and Gaussian mixture model clustering. Three distinct clusters were identified among bite marks attributed to this single documented interaction event, suggesting that variation in jaw orientation or repositioning may have occurred during the event. In this individual, wound closure occurred within approximately 4 weeks, providing a preliminary reference for the temporal interpretation of recent injuries, while melanized scars remained externally visible for more than 13 months. These observations provide a preliminary framework for evaluating pectoral fin bite marks as potential indicators of male–female interaction history in whale sharks, although validation across additional individuals is required. These findings provide a foundation for future studies integrating behavioral observations and scar-based assessments in both ex situ and in situ contexts.
