Application of nitrogen fertilizer is the main way to increase yield in forage production. However, excessive nitrogen application can increase nutrient leaching and cause environmental problems. In recent years, biochar has been used as a new soil amendment to slow and control the the release of nitrogen fertilizer which has achieved some initial effect. However, the application methods for biochar need to be optimized. The effects of biochar application at different depths on soil nitrogen and plant nitrogen distribution require further analysis. In this study, no biochar was used as the control, and 0－20 cm, 20－40 cm, and 40－60 cm deep treatments were set up to conduct field pot experiments. The results showed that compared with other treatments, surface application of biochar (0－20 cm) was more conducive to the increase of biomass, which could effectively increase the total biomass by 19.3%. The fruit biomass was 24.6% higher than that of the control treatment (P < 0.05). In terms of nitrogen accumulation, the nitrogen content of the aboveground component of maize (Zea mays) increased by 29.9%. The nitrogen content of the fruit and leaf sheath was significantly higher than that of the control treatment (P < 0.05), which was 29.7% and 42.3% higher than that of the control treatment, respectively. The nitrogen accumulation was consistent with the increase in biomass. In terms of soil nitrogen fixation, the soil nitrate nitrogen content at 0－20 cm in depth was higher than that of the other treatments. Meanwhile, a moderate increase in the application depth to 20－40 cm was more conducive to soil ammonium nitrogen fixation. All the biochar application depth treatments could promote root growth. The total root length at application at 20－40 cm in depth was significantly longer than that of the control treatment (P < 0.05). The total root surface area at an application depth of 0－20 cm was 49.1% higher than that of the control treatment. The total root volume was 215.1% higher than that of the control treatment (P < 0.05). Therefore, a biochar application depth of 0－20 cm could significantly improve root growth. This lead to an increase in nitrogen accumulation in the aboveground component, improving soil nitrate nitrogen retention capacity, and increasing forage maize yield. The results have provided a scientific basis for nutrient management of forage maize and strategic use of biochar.
Nitrogen mineralization in the soil of alpine grasslands on the Tibetan Plateau is a key process affecting the global carbon and nitrogen cycle, as well as the succession of grassland ecosystems. The aim of this study was to explore the effects of temperature and organic fertilizer additions on the nitrogen availability in the soil of these alpine grasslands. Through incubation experiments involving different temperatures (5, 15, and 25 ℃) and gradients of organic nitrogen addition (0, 15, 45, and 75 kg·hm−2), we analyzed changes in soil nitrogen mineralization rates, mineralization potential, and mineralization reaction rate. The results showed that soil nitrogen mineralization increased with rising temperature, with values significantly higher at 25 ℃ than at 15 ℃ and 5 ℃. Organic nitrogen addition (15-45 kg·hm−2) significantly increased the mineralization of ammoniacal nitrogen in the soil. However, further increasing the fertilization gradient to 75 kg·hm−2 reduced the nitrogen mineralization capacity. The soil nitrogen mineralization rate exhibited a single peak trend with increasing incubation time, and the addition of organic nitrogen could increase the nitrogen mineralization rate in alpine grassland soil, reaching its maximum under N45 treatment. Temperature determined the mineralization potential of nitrogen in alpine grassland soil, but did not significantly affect the mineralization rate, k. In summary, the addition of 15-45 kg·hm−2 of organic nitrogen and higher temperature conditions are beneficial for enhancing the mineralization capacity of rapidly available nitrogen in the soil of alpine grasslands on the Tibetan Plateau, providing a theoretical basis for grassland management and organic fertilizer application in alpine grassland regions.
With the continuous warming of the global climate, the ecological environment of the Qinghai–Tibet Plateau is highly fragile. Studying the effects of temperature and water conditions on the germination activities of wild herbage species in this region and selecting suitable grass species for ecological restoration based on these conditions is vital for stabilizing and restoring ecological barrier function. The PEG-6000 osmotic potential was used to simulate soil drought conditions, and the spring and summer temperature conditions were simulated at 10 ℃ and 20 ℃, respectively. The germination behavior of 14 native herbs on the Tibetan Plateau was studied under different temperature and water potentials. The results showed that the germination strategies of the plants tested were diversified. Most of the plants under different temperature and water potential showed that the germination rate increased or decreased along with the germination time and germination rate. 50% of the plants, comprising seven species, had significant germination time and weak responses in terms of their germination rate. 28.6% of the plants, comprising four species, showed considerable variation in the three. The germination rate and germination time of Anaphalis xylorhiza were significantly different in individual plants. Astragalus strictus showed a weak response and Silene moorcroftiana had an increased germination rate. Overall, the germination rate and germination rate of plant seeds increased significantly at 20 ℃, and increased with increasing water potential. The effect of temperature on germination rate was more significant than that of water potential. The germination time of 12 plant seeds at 20 ℃ was significantly shortened by 8–10 d. The plants of different families had different responses to the change in water and heat, and the plants from Compositae and Dianthus had a higher germination rate. Most of the plants could be used as established grasses for ecological restoration on the Qinghai–Tibet Plateau in summer when the moisture conditions are more suitable. Tibetan Artemisia wellbyi can also be established in summer, and Western Tibet Flysthopsis, Leontopodium nanum, and wood root sweet grass can be used as established grasses in spring.
To explore the effects of drought stress on the responses and adaptability of leaf photosynthesis and chlorophyll fluorescence in sainfoin (Onobrychis viciaefolia) seedlings, twenty different sainfoin seedling genotypes, derived from national and international strains, were used in this study. Water deficit stress was simulated with PEG-6000 at four osmotic potentials (−0.5, −1.0, −1.5 and −2.0 MPa, respectively), alongside a nil-PEG (CK) control treatment. Corresponding results demonstrated that drought stress inhibited the plant height of sainfoin seedlings. Additionally, with increasing drought stress, chlorophyll content, carotenoid content, transpiration rate, net photosynthetic rate, and stomatal conductance were reduced, while water use efficiency was improved. Initially, the stomatal limitation value increased with increasing drought stress, and then decreased, with a maximum value being observed at −1.0 MPa. Intercellular carbon dioxide concentration initially decreased and then increased, reaching a maximum value at −2.0 MPa; this indicated that non-stomatal factors limited photosynthesis. Although the non-photochemical quenching coefficient increased, the actual photochemical efficiency, maximum photochemical efficiency, electron transport rate, photochemical quenching coefficient, and photochemical quantum yield decreased with increasing drought stress. The comprehensive evaluation of the results based on the membership function value demonstrated that the novel P1, Onobrychis viciaefolia ‘Gansu’ sainfoin line and the 1626 genotype possessed the strongest drought tolerance, whereas genotypes 1668, 6, and 2049 possessed the weakest drought tolerance. Therefore, P1, Onobrychis viciaefolia ‘Gansu’ and 1626 sainfoin lines could be used for a variety of regional and production analyses in the future.
Elymus nutans is the first domesticated wild forage grass in Tibet. We selected three seed yield indices and seven agronomic traits in an effort to identify correlations between seed yield and agronomic traits of E. nutans‘Baqing’. We sought to identify correlations between seed yield and agronomic traits of E. nutans ‘Baqing’ by principal component analysis, typical correlation analysis, and a stepwise regression method. Correlation analysis revealed that the number of spikes per plant and yield per plant both correlated positively with plant height, number of tillers, and mean flag leaf length (P < 0. 01) with a high degree of significance. Typical correlation analysis revealed that the number of tillers and seed length structure coefficients increased with increases in the combined agronomic trait index variables V1 and V2, indicating that the number of tillers and plant height are the main factors determining seed yield from Plantago lanceolata pendants. Stepwise regression analysis gave highly significant (P < 0. 01) coefficients correlating single plant yield with number of single spikes and average number of grains per spike. The average number of grains per spike was significantly different from the partial correlations between number of single spikes, seed length, average number of nodules, plant height, and average flag leaf length (P < 0. 05). The partial correlation of the number of single spikes with seed length, awn length, and average number of nodules per spike was found to be significantly different (P < 0. 05).
To understand the effects of different utilization patterns of grassland resources on the species composition and diversity of different plant communities in the Songnen meadow steppe, this study investigated and analyzed two typical communities, Leymus chinensis and Arundinella hirta, under mowing and long-term grazing on the western Songnen Plain. We explored the effects of these two utilization patterns on the community composition of plant species, importance value, life form, quantitative characteristics and diversity. The results showed that there were 18 and 21 L. chinensis community species under mowing and long-term grazing, respectively, and the proportion of poaceae plants was the largest under both conditions at 33.3% and 45.45%, respectively. There were 39 and 32 A. hirta community species under mowing and long-term grazing, respectively, and the proportion of gramineous plants was 23.1% and 21.9%, respectively. Long-term grazing increased the proportion of hemicryptophytes in the L. chinensis community and therophytes in the A. hirta community, respectively, but it decreased the proportion of hemicryptophytes in the A. hirta community. Fine herbage species provided the largest variety in forage value in both communities under the two utilization patterns, accounting for 58.76% (L. chinensis mowing), 53.33% (L. chinensis long-term grazing), 46.56% (A. hirta mowing), and 46.63% (A. hirta long-term grazing), respectively. Long-term grazing significantly decreased the coverage, height, and biomass of the two plant communities (P < 0.05). Compared with mowing, the Shannon-Weiner and Simpson diversity indexes of long-term grazing plant communities showed an increasing trend. The Sörensen similarity index of both communities was higher under long-term grazing than under mowing. The similarity of plant species between the two communities was high, but the Cody index was higher under mowing. In addition, changes in the species composition of both communities were greater under mowing. These results show that mowing and grazing have different effects on the composition and diversity of different plant communities in the Songnen meadow steppe. Reasonable and appropriate utilization and protection measures will be conducive to the sustainable use of grassland resources.
为研究处于宁夏不同气候条件草地百里香(Thymus monogolicus)不同部位的鲜、干材料挥发性有机物的组成及其差异,本研究于10月上旬分别在宁夏盐池麻黄山荒漠草地、固原云雾山典型草地与海原南华山草甸草地采集百里香地上植株,采用气相色谱-质谱联用技术(GC-MS)对其挥发性成分进行分离鉴定,利用面积归一化法确定各成分的相对含量.结果表明:3种类型草地百里香不同部位鲜、干样品中共检测出339种挥发性有机物,分属于烃类(125种)、醇类(70种)、酯类(29种)和其他类化合物(115种).对于挥发性成分种类数量,草甸草地百里香挥发性有机物数量大于荒漠草地和典型草地,鲜样多于干样.挥发性成分中烃类物质相对含量最多,主要以萜烯类物质对伞花烃和γ-萜品烯为主,分别为14.24％～42.24％和3.30％～37.35％,对伞花烃在草甸草地的相对含量多于典型草地和荒漠草地,在嫩茎中多于木质化茎和叶片,而在鲜样与干样中的相对含量相当.酚类、醇类及其他挥发性有机物主要有香芹酚、桉树醇、芳樟醇及2-异丙基-4-甲基苯甲醚,香芹酚的相对含量在荒漠草地最高,达20.61％,桉树醇和芳樟醇在草甸草地的相对含量最高,分别为9.68％和12.99％.综上,采样地气候条件、样品处理及不同部位对百里香释放的挥发性物质种类和含量具有一定影响,干燥样仍能较好地保持百里香的主要成分组成,研究结果可为宁夏野生百里香资源的开发应用提供科学依据.
致死粒线虫(Anguina funesta)是具有重要经济意义的粒线虫,被多国列入检疫性有害生物名录.2021年,上海口岸从来自美国的多花黑麦草(Lolium multiflorum)种子中截获一种粒线虫属线虫,但在该样品中仅分离得到幼虫,无法准确鉴定.本研究运用形态学与分子生物学相结合的方法对分离得到的幼虫进行鉴定.采用28S通用引物扩增该线虫序列,测序获得742 bp的序列,与GenBank中登录的致死粒线虫相似性达100％;采用ITS通用引物对ITS区扩增并测序,获得778 bp的序列,与GenBank中登录的致死粒线虫相似性达99.86％～100％.基于28S及ITS区序列构建的系统进化树均显示,该线虫与致死粒线虫位于同一进化枝上.综合形态学特征及分子鉴定结果,将该群体鉴定为致死粒线虫.这是我国口岸首次报道截获该线虫,本研究将为口岸检疫部门鉴定该线虫提供技术参考.
采用盆栽试验研究了不同光周期(8、10、12、14、16 h·d-1)对宁夏无芒稗(Echinochloa crusgalli)生理特性的影响,通过揭示光周期对其生长发育的调控机理,寻找最适光周期,以期为其科学引种及高产栽培提供指导.结果表明:1)在16 h·d-1光周期处理下,宁夏无芒稗的生育期显著延长.2)随光照时间延长,其地上生物量呈上升趋势;穗重呈"倒V"型变化.3)随光照时间延长,其抗氧化酶活性呈先降后升的趋势;叶绿素含量、净光合速率等呈相反变化;随着生育期的推进,光合色素含量呈下降趋势.综上所述,12 h·d-1光周期处理促进了宁夏无芒稗光合色素的合成,增强了叶片光合能力,促进了植株的生长发育.因此,应将宁夏无芒稗引种种植在光周期12 h·d-1的地区,在较短时间内通过适宜的光照时间更大程度地提高牧草产量.
作为城市绿地生态系统中的主要景观类型,城市草坪频繁受到人为管理措施的强烈干扰.本研究以沟叶结缕草(Zoysia matrella)为对象,通过控制3个灌溉水平(I1:4.00 L·m-2、I2:5.71 L·m-2、I3:7.43 L·m-2)和3个修剪水平(M1:不修剪、M2:留茬2 cm、M3:留茬6 cm)的盆栽试验,探究不同灌溉和修剪水平对草坪草生长、土壤无机氮及氮素表观平衡的影响.结果表明:相同灌溉水平下,M2处理草坪草地上生物量显著低于M1和M3处理,但M2处理草坪草地上部分和地下部分氮含量均显著高于M1处理(P<0.05).在草坪草生长末期,相同灌溉水平下M2处理土壤无机氮(Nmin)含量较M1、M3处理下降了0.6％～54.1％.各处理淋溶液中Nmin均以铵态氮(NH4+-N)为主要形式,不过,Nmin淋失量仅占氮输出总量的0.4％～1.3％.相同灌溉水平下,M2处理的氮平衡相较于M3处理表现出更为明显的盈余特征(P<0.05),但M3处理在高灌溉量下会更趋于氮素收支平衡.综上可知,重度修剪(M2处理)显著抑制了草坪草生长,增加了氮素损失风险,但却提高了草坪草氮素吸收量,因此,在本研究条件下,建议灌溉量(5.71～7.43 L·m-2)略高于一般灌溉水平,同时适度修剪(保持留茬高度为6 cm),既可以保证草坪草良好的生长状况,也可维持土壤氮素基本收支平衡,提高氮素利用效率.
近年来,随着机器学习算法的发展,国内外专家学者致力于利用机器学习模型展开土壤盐渍化遥感相关研究,并取得了丰硕成果.本文利用文献计量可视化软件CiteSpace,对近十年来基于机器学习的土壤盐渍化遥感建模研究进行分析,确定了研究主题和热点,从机器学习算法、建模特征变量以及模型评价等方面评述研究进展,并针对当前研究热点对目前研究的局限性与发展趋势进行讨论.主要结论:1)机器学习算法在土壤盐渍化遥感建模中发挥着至关重要的作用,主要研究主题有机器学习算法及其精度研究、建模特征变量选择研究、遥感数据源选择对模型的影响研究、土壤盐渍化研究区域选择和基于机器学习的土壤盐渍化数字制图应用研究.2)目前的研究热点为辅助变量作为特征变量在模型构建中的应用、实测光谱数据与多源遥感光谱数据结合以及最佳机器学习算法选择.3)以2018年为节点,研究进展分为起步阶段和高速发展阶段,目前仍存在需要解决的挑战以提高模型的准确性.未来的研究方向应集中在云平台和机器学习在土壤盐碱化大范围、长期监测中的应用.
过氧化氢酶(CAT)是可清除生物体内多余的过氧化氢(H2O2)并将其还原成水和氧气的氧化还原酶,在植物响应胁迫的过程中发挥多种功能,保护植物细胞免受活性氧毒害.目前,尚缺乏对空心莲子草(Alternanthera philoxeroides)CAT基因家族成员的系统研究.本研究通过生物信息学分析,从空心莲子草中鉴定到20个ApCAT基因,根据系统发育关系将其依次命名为ApCAT1至ApCAT20.特征分析表明,所有ApCATs均为亲水性蛋白.亚细胞定位预测发现,所有ApCATs均预测定位于过氧化物酶体,个别ApCATs也预测有定位线粒体、叶绿体和细胞核的可能.表达模式分析表明,ApCAT成员受纬度、海拔、温度、光照、水分条件等的影响较小,表达水平相对稳定.除草剂药效测试发现,20％氯氟吡氧乙酸和13％噁草酮的防治效果最好;结合定量PCR分析发现,ApCAT家族成员在空心莲子草响应除草剂处理过程中显著差异表达,参与对除草剂胁迫的应答过程.本研究系统分析了ApCATs家族成员的基本特征和表达模式,揭示了其除草剂诱导表达特征,为进一步研究ApCATs在空心莲子草响应除草剂胁迫中的生物学功能奠定了基础.
为探讨宁夏半干旱区紫花苜蓿(Medicaco sativa)种子田高效生产的最佳播种量,采用单因素随机区组设计,研究不同播种量(S1:1.2 kg·hm-2,S2:1.8 kg·hm-2,S3:2.4 kg·hm-2,S4:3.0 kg·hm-2,S5:3.6 kg·hm-2)对紫花苜蓿种子生产性能和构成因素的影响.结果表明,播种量对紫花苜蓿株高、一级分枝数、每生殖枝花序数、每花序小花数、每花序结荚数、单位面积生殖枝条、每荚种子数、千粒重、实际种子产量和表现种子产量均有显著影响(P<0.05),其中单位面积生殖枝数、每生殖枝花序数、每花序结荚数、每荚种子数及千粒重均在S4播量下达到最高,分别为177.09、23.35、12.56、6.16和1.88 g;株高和每花序小花数在S2播量下达到最大,分别为87.81 cm和18.18;以上指标均随播种量的增加呈先增加后降低趋势.表现种子产量和实际种子产量均在S4处理下最大,分别为5993.39和630.68 kg·hm-2,较S1处理提高了180.25％和38.41％.相关性分析表明,每生殖枝花序数与每花序小花数和种子产量间均极显著正相关(P<0.01).主成分分析显示,每生殖枝花序数和实际种子产量的贡献率最大,每荚种子数次之,株高的贡献率最小,综合排名由高到低为S4>S3>S2>S5>S1.建议在宁夏半干旱区紫花苜蓿种子田生产的最佳播种量为3.0 kg·hm-2.
为探究菊芋(Helianthus tuberosus)对不同浓度盐胁迫初期的响应机制,揭示生物炭对不同浓度盐胁迫初期菊芋渗透调节作用和抗氧化系统的短期影响,本研究采用盆栽试验,以菊芋块根为材料,设置8种处理:无盐胁迫空白对照(CK)、1.67 g·kg-1盐胁迫(Y1)、3.35 g·kg-1盐胁迫(Y2)、5.02 g·kg-1盐胁迫(Y3)、无盐胁迫+5％生物炭(S0)、1.67 g·kg-1盐胁迫+5％生物炭(S1)、3.35 g·kg-1盐胁迫+5％生物炭(S2)、5.02 g·kg-1盐胁迫+5％生物炭(S3),分析不同浓度盐胁迫初期生物炭添加量对菊芋株高、地径、渗透调节物质含量、丙二醛(MDA)含量及抗氧化酶活性的影响.结果表明:1)菊芋能在0～1.67 g·kg-1土壤含盐量范围内正常生长,当土壤含盐量超过3.35 g·kg-1时会形成盐胁迫,显著抑制菊芋生长(P<0.05),可溶性蛋白(SP)、可溶性糖(SS)、脯氨酸(Pro)等渗透调节物质含量和MDA含量均显著增加(P<0.05),过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等酶活性显著提高(P<0.05).2)生物炭可有效提高菊芋对盐胁迫的抵抗能力,显著降低MDA含量,缓解盐胁迫对菊芋生长抑制作用,其中,相比5.02 g·kg-1盐胁迫,生物炭处理的SP、SS、Pro增量分别下降了161.76％、16.83％、55.48％,MDA增量下降了61.83％,POD、SOD、CAT活性也分别降低了71.85％、66.25％、340.05％.因此,添加生物炭能有效缓解由盐胁迫导致的膜脂过氧化作用,提高抗氧化能力,增强菊芋对盐胁迫的抵抗能力.
为探究覆盖度变化对植物光谱反射率的影响,实现覆盖度和生物量的高精度反演,以伊犁绢蒿(Seriphidium transiliense)为对象,通过室内控制试验获取不同覆盖度下植物光谱反射率,采用最大归一法、一阶微分和二阶微分变换分析其反射率变化,利用归一化植被指数(NDVI)、比值植被指数(RVI)、增强型植被指数(EVI)和土壤调节型植被指数(SAVI)共4种植被指数对覆盖度与生物量进行反演,探讨反演精度对覆盖度变化的响应.结果表明:伊犁绢蒿植物种群的冠层光谱反射率在可见光波段随覆盖度增加逐渐减小,在近红外波段逐渐增加,在680 nm处形成植物特有的红边效应,最大归一化和微分变换突出了植物的光谱特征;光谱反射率差值在可见光波段随覆盖度增加基本稳定;利用植被指数逐级反演覆盖度与生物量时,精度随覆盖度增加整体呈上升趋势;利用土地利用/覆盖变化(LUCC)分覆盖度反演时,提高了覆盖度大于20％的伊犁绢蒿种群的数量特征反演精度,且最佳指数为SAVI.
草畜平衡管理是保护草原的重要措施,原《草畜平衡管理办法》(以下简称《办法》)被废止后,中央层面的具体制度内容存在空白,亟需补充相关制度依据.总结原《办法》和各地规定,草畜平衡管理面临法律依据缺位、草畜平衡内涵界定不清、草畜平衡标准制定的公众参与度不足、行政处罚方式和标准不统一等问题.未来立法应当坚持生态保护优先、兼顾牧民生产生活的原则,禁牧休牧、草畜平衡和生态补偿制度协调配合,转变草畜平衡管理理念.在具体制度设计上,应当提升规范层级,制定《草原禁牧休牧和草畜平衡管理条例》;界定管理对象,明确草畜平衡管理范围;增加听证制度,保障标准制定中的公众参与机制;明确补偿和处罚制度,优化激励和制裁措施.
辣木(Moringa oleifera)是优质木本蛋白饲料,已广泛应用于家畜、家禽和水产动物饲喂中,在我国传统饲料资源日益紧张的情况下,具有广阔的开发前景.本文综述了辣木的营养成分及其在畜禽和水产动物饲喂中的效果,以期为辣木资源的深入开发利用提供参考.分析得知,辣木含有丰富的矿物质、蛋白质、氨基酸、维生素及黄酮、多酚等生物活性物质;其青贮或作为饲料添加饲喂畜禽,不仅能提高畜禽生长速度、缩短出栏时间、降低饲养成本,改善畜禽肉、蛋、奶品质,还可提高畜禽抗氧化能力、改善血液指标、提高机体免疫力.未来辣木资源开发时,需进行不同生态区辣木适应性评价,辣木机械化收获技术研制,辣木饲料精深加工技术研究,不同畜禽饲喂方法、饲料配方和饲喂效果评价,水产养殖中适宜辣木添加量确定等.
构建全员、全过程、全方位"三全育人"课程体系是高校通过"课程育人"实现"立德树人"根本任务的主要途径."牧草与草坪草种子学"是高校草业科学专业的一门重要专业课,在草种专业理论知识和实际生产应用技术教育中发挥着重要作用.基于"三全育人"理念,本文围绕课程教育重要性、课程育人目标(专业知识、专业技能、综合素质)和课程教育体系构建(教学大纲、参考教材、教学内容设计、教学方法与教学过程、教育成效考核体系)进行了初步探索与实践,将课程育人元素有机融入专业知识技能教育和考核评价环节,旨在发挥专业课程育人功能,为社会培养输出具备专业知识扎实、实践技能熟练、综合素质高、使命担当意识强的复合型草业科学专业人才,助力草种业振兴和草产业健康高质量发展.
为探究雾冰藜(Bassia dasyphylla)对干旱胁迫的结构、生理和分子响应机制,对不同干旱胁迫梯度处理的叶片进行解剖结构、生理指标及转录组学测定.结果表明:干旱胁迫导致贮水组织厚度显著降低(P<0.05),叶厚和表皮厚在干旱胁迫7 d时显著增大(P<0.05),分别增加24.32％和84.58％;脯氨酸含量在干旱胁迫21 d最高(P<0.05),H2O2含量在胁迫14和21 d显著增多(P<0.05),超氧化物歧化酶(SOD)活性先降后升,在胁迫21和28 d显著升高,过氧化物酶(POD)活性在胁迫14 d最大(P<0.05),为38.08 U·g-1.与0 d相比,干旱胁迫7、14、21和28 d的差异表达基因(DEGs)分别有1860、2781、1550和819个;DEGs显著富集在有机氮复合代谢过程、氧化磷酸化、防御反应、ATP代谢过程等GO条目中;DEGs显著富集在氧化磷酸化、蛋白酶体、核糖体和剪接体等KEGG通路中.非生物胁迫相关通路DEGs分析表明,胁迫感知受体激酶(热激蛋白、干旱/盐胁迫蛋白等),抗氧化酶(CCS、POD等)、信号通路(Ca2+、MAPK等)、转录因子(ERF、bZIP、MYB等)、激素信号通路、细胞壁合成、蛋白质降解和次级代谢物等通路基因参与干旱胁迫响应调节.研究结果可为今后雾冰藜抗旱基因资源挖掘和利用提供参考.
