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    专

    一种区域耦合气候延伸期预测方法和系统

    PL1088129B2
    发明人
    李清泉, 胡晓明, 张晓曼, 董李丽, 王铭昊, 陆戈曼, 李珈莹
    受让人
    -
    申请人
    SUZHOU HOLINK TECH CO LTD
    申请号
    225834
    申请日
    1997-02-23
    公开(公告)号
    PL1088129B2
    公开(公告)日
    1998-08-04
    IPC分类号
    H01L039/22H01L039/24B05D005/12
    CPC分类号
    -
    优先权号
    231495
    711681
    CA00069
    优先权日
    2002-01-17
    2002-01-17
    2002-01-17
    摘要

    NOVELTY - The structure (300) has plasma display panel (110) with a front glass and a rear glass. The panel displays images through the front glass. The rear glass is inter-spaced with the front glass. A plasma is enclosed between the rear and front glasses. A filter (305) is directly formed upon the front glass so that the filter is enforced and supported by the front glass to remove an original glass layer within the filter.

    USE - Used in a plasma TV (claimed).

    ADVANTAGE - The filter is directly formed upon the front glass so that the filter is enforced and supported by the front glass to remove the original glass layer within the filter, thus facilitating the plasma TV to be thinner, lighter, and cheaper with low manufacturing cost and also with a safety consideration.

    DETAILED DESCRIPTION - An INDEPENDENT CLAIM is also included for a plasma TV.

    DESCRIPTION OF DRAWING(S) - The drawing shows a simple illustration for a side cross-sectional view of a display panel structure.

    Plasma display panel (110)

    Display panel structure (300)

    Filter (305)

    Color compensating layer (320)

    Hardening arrangement layer (330)

    权利要求
    1.用于检测猫支原体和猫衣原体的引物探针组合物,其特征在于:包括用于检测猫支原体的上游引物、用于检测猫支原体的下游引物、用于检测猫支原体的荧光探针、用于检测猫衣原体的上游引物、用于检测猫衣原体的下游引物、用于检测猫衣原体的荧光探针、外参的上游引物、外参的下游引物以及外参的荧光探针;所述用于检测猫支原体的上游引物:序列如SEQ ID NO.1所示;所述用于检测猫支原体的下游引物:序列如SEQ ID NO.2所示;用于检测猫支原体的荧光探针:序列如SEQ ID NO.3所示;用于检测猫衣原体的上游引物:序列如SEQ ID NO.4所示;用于检测猫衣原体的下游引物:序列如SEQ ID NO.5所示;用于检测猫衣原体的荧光探针:序列如SEQ ID NO.6所示;所述外参的上游引物:序列如SEQ ID NO.7所示;所述外参的下游引物:序列如SEQ ID NO.8所示;所述外参的荧光探针:序列如SEQ IDNO.9所示。
    2.根据权利要求1所述的用于检测猫支原体和猫衣原体的引物探针组合物,其特征在于:所述用于检测猫支原体的荧光探针:5’端标记为报告基团6-FAM,3’端标记为猝灭基团BHQ1;所述用于检测猫衣原体的荧光探针:5’端标记为报告基团ROX,3’端标记为猝灭基团BHQ2;所述外参荧光探针:5’端标记为报告基团VIC,3’端标记为猝灭基团BHQ1。
    3.用于检测猫支原体和猫衣原体的试剂盒,其特征在于:包括阳性对照、阴性对照、用于检测猫支原体和猫衣原体的引物探针组合物、酶和Buffer;所述用于检测猫支原体和猫衣原体的引物探针组合物:序列如SEQ ID NO.1- SEQ ID NO.9所示。
    4.根据权利要求3所述的用于检测猫支原体和猫衣原体的试剂盒,其特征在于:所述阴性对照为TE Buffer。
    5.根据权利要求3所述的用于检测猫支原体和猫衣原体的试剂盒,其特征在于:所述阳性对照:含猫支原体人工合成质粒与猫衣原体人工合成质粒的浓度均为1×10 6 copies/mL。
    6.根据权利要求3所述的用于检测猫支原体和猫衣原体的试剂盒,其特征在于:所述试剂盒采用的荧光定量PCR反应体系如下:采用25μL的反应体系,其中有5μL Taq酶 Buffer、1μL Taq DNA聚合酶,靶标引物探针终浓度均为2μM,外参引物探针终浓度均为1μM, DEPC处理水10.25μL,模板量为5μL。
    7.根据权利要求3所述的用于检测猫支原体和猫衣原体的试剂盒,其特征在于:所述试剂盒采用的荧光定量PCR反应程序为:50℃,2min;95℃,预变性10min;94℃,变性15s,60℃,退火和延伸共40s,循环45次。
    说明书
    [0001]FIG. 1 is a perspective view of a bottle for water dispensers according to our design. FIG. 2 is a front view of the bottle for water dispensers. FIG. 3 is a left side view of the bottle for water dispensers. FIG. 4 is a right side view of the bottle for water dispensers. FIG. 5 is a back view of the bottle for water dispensers. FIG. 6 is a top view of the bottle for water dispensers; and, FIG. 7 is a bottom view of the bottle for water dispensers.
    同族专利
    暂无同族专利