Grapevine is one of the most economically important fruit crops cultivated worldwide. In recent decades, the increasing frequency and intensity of high temperature (HT) events have posed significant challenges to viticulture. However, previous researches have mostly focused on heat-sensitive cultivars response to HT, and were conducted in artificially controlled growth chamber, which limited deep understanding of thermotolerance mechanism of grape. In this study, we conducted physiological and transcriptomic analyses of berries and leaves at different development stages in 'Guipu No.1' (GP, a hybrid of wild V. quinquangularis and V. vinifera, heat tolerant) and 'Cabernet Sauvignon' (CS, a cultivar from V. vinifera, heat sensitive) grapevines exposed to natural subtropical HT. Under HT conditions, GP maintained higher total anthocyanins content and photosynthetic capacity compared to CS. Differential expression was observed in genes involved in both the light and dark reactions of photosynthesis, as well as in structural genes and transcript factors associated with anthocyanins biosynthesis. Notably, protein processing in endoplasmic reticulum emerged as a key biological process potentially underlying thermotolerance differences. Based on the above results, we also identified and characterized a class B Heat Shock Factor, HSFB3, which was significantly up-regulated in GP but not in CS under HT. The GP-derived VqHSFB3 and CS-derived VvHSFB3 differed by only one amino acid and exhibited similar transcriptional repression activity. Overexpression of HSFB3 in grapevines indicated that it acts as a negative regulator of thermotolerance. Together, these findings provide new insights into the molecular and physiological basis of grapevine thermotolerance and offer potential targets for breeding heat-tolerant cultivars.

[Objective]In recent years,with the promotion of rain-shelter cultivation and double cropping techniques,grape production in hot zones has achieved off-season and high-efficiency production.However,hot-region grapes face problems such as poor flower bud differentiation,uneven germination,and"flower running",which are the main constraints on stable and high-quality production.This study aims to systematically investigate and evaluate the inflorescence attachment characteristics and development quality of 98 grape germplasms in hot zones Guangxi,clarify the adaptability differences among different germplasms in hot zones,and provide theoretical basis and technical guidance for variety breeding and production management.[Method]This study investigated and evaluated the germination rate,flower bud rate,shoot formation rate,number of inflorescences per shoot,inflorescence attachment node,inflorescence type and quality of 98 grape germplasms in hot zones Guangxi for two consecutive years,covering European,American,and European-American hybrids.It deeply analyzed the quality differences and patterns among different varieties and inflorescence attachment nodes.[Result]Under the treatment of hydrogen cyanamide,all 98 germplasms could germinate and form shoots smoothly,among which 92 varieties could form normal inflorescences.The average germination rate was 90.42％,the shoot formation rate was 92.13％,the flower bud rate was about 61.06％,and the average number of inflorescences per shoot was 1.67.However,abnormal inflorescences were quite prominent,with a total proportion of 40.56％.This study classified abnormal inflorescences into three major types:tendrils type,differentiation cessation type and trophic tissue type.Among them,the tendril type was the most common,further divided into one to five tendril types,and all investigated varieties had two tendrils type inflorescences;the differentiation cessation type included single head type(only scales or death point),tendrils death point type,and branch death point type;the trophic tissue type included branch tendril and leaf tendril types.Shine Muscat showed all abnormal types and were representative materials of high sensitivity.Among the 8 investigated nodes,the inflorescence attachment was most concentrated at the 3rd and 4th nodes,accounting for 27.99％and 27.06％of the total inflorescences,respectively.The abnormal inflorescence rate at the 3rd node was the lowest,only 17.22％.Further population analysis indicated that V vinifera L.×V.labrusca L.had higher flower bud rates(68.60％),more inflorescences per shoot(about 1.8),and higher normal inflorescence rates than V vinifera(flower bud rate 46.59％).Moreover,varieties with high germination rates,flower bud rates,and shoot formation rates had more inflorescences per shoot and significantly lower abnormal inflorescence rates.[Conclusion]The attachment node of inflorescences significantly affects their quality performance.Among them,the middle nodes,especially the 3rd node,had the lowest abnormal inflorescence rate.Therefore,in production,for varieties prone to abnormal inflorescences,it is recommended to prioritize the retention of inflorescences at the 3rd node.Varieties with high germination rates,flower bud rates,and shoot formation rates and more inflorescences per shoot are more likely to obtain normal and high-quality inflorescences.Different grape populations show significant differences in inflorescence development and abnormal occurrence.V vinifera L.×V.labrusca L.perform better in hot zones,with higher flower bud rates and normal inflorescence proportions.They are recommended as the preferred germplasm resources for cultivation in hot zones.

With the increasing challenges posed by global warming and climate change, heat stress has become a significant threat to the sustainable production of grapevines. However, the genetic basis of grapevine thermotolerance remains poorly understood. Here, we combine genome-wide association study with transcriptomic profiling to identify TTC4 (thermotolerance on chromosome 4), a gene encoding a WRKY transcription factor, as a key determinant of thermotolerance in grapevine. TTC4 directly activates two thermotolerance-related genes, HSP18.1 and APX3. We also identify a heat-suppressed repressor SPL13 (SQUAMOSA-promoter binding protein-like 13) that cannot bind to the GTAT element (TTC4T(7631)) in intron 2 of TTC4, but can bind to the natural variant, GTAC (TTC4C(7631)). Grapevine accessions with TTC4C/C(7631) genotype exhibit significantly lower thermotolerance compared to those with the TTC4T/T(7631) and TTC4C/T(7631) genotypes. This fine-tuned regulation contributes to thermotolerance divergence among grapevine populations. The TTC4T(7631) haplotype holds significant potential as a genetic resource for breeding thermotolerant grapevine varieties.

Powdery mildew (PM) disease of grapevine is a significant production problem in most areas of the world in which grapes are grown. The disease is caused by the fungus Erysiphe necator Schw. [syn. Uncinula necator (Schw.) Burr.]. Members of the WRKY transcription factor are key regulators of plant immunity to PM. In this study, the level of VqWRKY2 transcript was quickly induced in response to E. necator inoculation and levels increased in most organs and tissues. We isolated VqWRKY2 from an accession of the Chinese wild grape, Vitis quinquangularis, Shang-24, which shows high resistance to PM. The results indicate that VqWRKY2 localized in the nucleus and showed transcriptional activation in yeast is a Group I protein of the WRKY family and is similar to Citrus sinensis CsWRKY2. We reveal that overexpression of VqWRKY2 in Arabidopsis enhances resistance to PM that is associated with increased cell death and expression of nonexpressor of pathogenesis-related genes 1 (NPR1) in the salicylic acid (SA) signaling pathway. The physical interaction between VqWRKY2 and VqWRKY46 was demonstrated by the Yeast Two-Hybrid, Bimolecular Fluorescence Complementation and Pull-down assay. Our results contribute to the development of the theoretical basis which will help support further research on the development of new disease-resistant genotypes of grapevine. Overexpression of grapevine VqWRKY2 in Arabidopsis thaliana enhances resistance to powdery mildew by increasing cell death and activating the SA signaling pathway.

为探究"夏黑""阳光玫瑰"葡萄在摘心处理后对叶片光合和叶绿素荧光特性的响应,为这两个葡萄品种的栽培研究提供理论依据,以当年生"夏黑""阳光玫瑰"葡萄单株盆栽苗为试验材料,采用顶芽摘心技术进行改善植株光合效率的试验,以不摘心为对照,测定摘心20～21d后标记叶的光合参数和叶绿素荧光参数.结果表明,摘心后,"夏黑""阳光玫瑰"葡萄的净光合速率(Pn)均显著增加,分别比对照增加16.8％、35.7％;气孔导度(Gs)分别比对照增加31.7％、14.2％;胞间CO2浓度(Ci)分别比对照提高8.6％、5.3％;蒸腾速率(Tr)分别比对照提高12.6％、39.8％;摘心后水蒸气压亏缺(VPD)分别比对照降低26.3％、23.6％.摘心有助于提高葡萄叶片的Pn、Gs、Ci、Tr,降低葡萄叶片的VPD.摘心后,两种葡萄叶片的初始荧光(F0)降低,热耗散量子比率(F0/Fm)显著低于对照4.34％、3.66％,而光系统Ⅱ最大光能转换效率(Fv/Fm)分别显著高于对照处理0.93％、0.87％.光合参数Ci、Gs、VPD、Pn、Tr、WUE相互之间存在极显著相关性,叶绿素荧光参数F0、Fm、F0/Fm、Fv/Fm相互存在极显著相关性,但光合参数中只有VPD、Pn、Tr与叶绿素荧光参数显著相关.

The fast-growing demand for seedless table grapes has attracted the attention of scientists for the development of new seedless cultivars. Various genes and pathways have been identified which affect seedlessness. However, the detail of the mechanism(s) regulating seedless traits in grape is still unclear, and genes related to seedlessness in grape require further study. Transcriptomic and genomic analyses of Homeobox (HB) transcription factors have suggested the involvement of HB genes, especially of HB-KNOX members, in grape seed development. Here, we functionally characterize VvHB63 gene in grape and report its role in fruit and seed development. VvHB63 showed higher expressions levels in the chalaza and integument of ovules in seedless grapes, than in seeded ones. However, no differences were observed in the sequences of seedless and seeded grape cultivars. In situ hybridization (ISH) analysis showed that VvHB63 gene was expressed in the episperm cells and ovules of 'Thompson Seedless'. Conserved domains KNOX1 and KNOX2 were important for the interaction of VvHB63 with VvHB06. Heterologous over-expression of VvHB63 (35 S::VvHB63-OE) in tomato induced smaller fruits and seeds than in wild type or SlTkn1-KO. The synergistic cooperation between VvHB63 and related proteins play an important role in ovule development.

为了筛选适宜广西种植的无核葡萄品种,广西壮族自治区农业科学院葡萄与葡萄酒研究所引进紫甜无核葡萄品种开展栽培试验,观察其植物学特性、物候期、生长结果习性、果实品质及抗病性等表现,并研究配套栽培技术.2年的观察结果表明:紫甜无核葡萄品种在广西南宁综合性状表现优良,中熟,7月上旬成熟,果粒大小一致,果粒紫黑色且着色均匀,不裂果,可溶性固形物含量18.00％,果实挂树期长,适合在广西南宁及气候类似地区避雨栽培条件下种植.

Vitis adenoclada is a wild grape unique to China. It exhibits well resistance to heat, humidity, fungal disease, drought, and soil infertility. Here, we report the high-quality, chromosome-level genome assembly of GH6 (V. adenoclada). The 498.27 Mb genome contained 221.78 Mb of transposable elements, 28,660 protein-coding genes, and 481.44 Mb of sequences associated with 19 chromosomes. GH6 shares a common ancestor with PN40024 (Vitis vinifera) from approximately 4.26-9.01 million years ago, whose divergence occurred later than Vitis rotundifolia and Vitis riparia. Widely-targeted metabolome and transcriptome analysis revealed that the profiles and metabolism of phenolic compounds in V. adenoclada varieties significantly were differed from other grape varieties. Specifically, V. adenoclada varieties were rich in phenolic acids and flavonols, whereas the flavan-3-ol and anthocyanin content was lower compared with other varieties that have V. vinifera consanguinity in this study. In addition, ferulic acid and stilbenes content were associated with higher expressions of COMT and STSs in V. adenoclada varieties. Furthermore, MYB2, MYB73-1, and MYB73-2 were presumably responsible for the high expression level of COMT in V. adenoclada berries. MYB12 (MYBF1) was positively correlated with PAL, CHS, FLS and UFGT.Meanwhile, MYB4 and MYBC2-L1 may inhibit the synthesis of flavan-3-ols and anthocyanins in two V. adenoclada varieties (YN2 and GH6). The publication of the V. adenoclada grape genome provides a molecular foundation for further revealing its flavor and quality characteristics, is also important for identifying favorable genes of the East Asian species for future breeding.

目的 调查福建地区猪源大肠杆菌耐药基因oqxAB流行性及阳性菌中其重要耐药基因的流行情况,以期为本地区抗菌药的合理应用提供依据.方法 采集腹泻仔猪肛门拭子分离大肠杆菌,通过琼脂二倍稀释法测定分离株的药物敏感性,并用PCR法检测PMQR(包括oqxAB)、ESBLs及其他耐药基因;对oqxAB阳性菌进行接合转移试验,测定接合子的药物敏感性变化;对阳性菌的种系发育及毒力基因分布进行测定.结果 本研究共分离鉴定大肠杆菌133株,分离株对磺胺甲噁唑/甲氧苄啶的耐药率最高(90.2％),对多粘菌素的耐药率最低(24.8％),其余药物的耐药率在26.3％～85.7％;耐药基因检测结果显示,oqxAB基因检出率为29.3％(39/133),在oqxAB阳性菌中还检测出floR(76.9％)、qnrS(71.8％)、CTX-M-1G(59.0％)、mcr-1(56.4％)、fosA3(28.2％)和 acc(6')-Ib-cr(23.1％)等耐药基因,其中 59.0％携带 6 种以上耐药基因,以 oqxA+oqxB+qnrS+CTX-M-1G+mcr-1+floR基因型最为流行(38.5％);种系发育显示分离株主要分布在A组(46.2％),毒力基因主要分布在B2和D组,其毒力基因平均个数分别为3.43和2.64.69.2％(27/39)oqxAB阳性菌成功将耐药质粒传给受体菌(C600),其接合子的MIC值较受体菌均有不同程度的提高.结论 福建地区猪源大肠杆菌中.qxAB耐药基因携带率较高,在oqxAB阳性菌中携带多种耐药基因,种系发育主要分布在共生型(A组),毒力基因主要分布在B2和D群;携带.qxAB基因的阳性质粒可通过接合转移的方式进行水平传播.

采用气相-离子迁移谱法(GC-IMS)比较110R、5BB、3309m、SO4砧木嫁接及自根苗瑞都红玉葡萄挥发性成分的差异,评价砧木嫁接葡萄果实香气品质,为瑞都红玉葡萄生产中砧木选择提供理论依据.结果表明:GC-IMS共检测出46种挥发性成分,鉴定25种,包括醇类、醛类、酯类、酮类等,其中以醇类为主.不同砧木嫁接瑞都红玉葡萄具有该品种玫瑰香型的典型香味特征,同时存在一定差异性.芳樟醇、辛醛、异丁醛、2-己烯醇、己醛、戊醛、异丙醇、庚醛等是共有的主要挥发性成分,赋予葡萄果实玫瑰、柑橘类的花香和果香等香气特征.5BB、3309m、SO4砧木样品中芳樟醇含量较高,使其具有较浓郁的玫瑰花香;同时SO4砧木样品中还具有甜香、果香等香气特征;5BB砧木样品具有草本植物的青香特征;110R砧木样品具有显著的柑橘类果香特征.基于主成分(PCA)分析,各砧木样品挥发性成分之间可以显著区分.SO4砧木嫁接瑞都红玉葡萄具有较好的综合香气品质.GC-IMS分析方法可以高效快速评价砧木嫁接葡萄果实香气特征,为嫁接砧木选择提供理论依据.

为了充分利用广西及南方地区的温光资源,引种适合一年两收的无核、红色、具香味的葡萄新品种,本试验引进郑艳无核葡萄新品种在广西南宁种植,观察其植物学特性、物候期、生长结果习性、果实性状及抗病性等表现,并研究其一年两收栽培技术.经过4年的引种观察,郑艳无核葡萄在广西南宁可成功实现一年两收,表现为红色、无核、早熟、果穗中等、不易裂果、着色均匀、有香气、稳产等特点.4年引种观察期间,一茬果每667 m2产量最多为708.14 kg,二茬果每667 m2产量最多为578.02 kg;一茬果可溶性固形物含量最高为22.97％,二茬果可溶性固形物含量最高为23.71％;一茬果在6月中旬至月末果实完全成熟,二茬果在11月底至12月初果实完全成熟.郑艳无核葡萄在广西南宁引种表现优良,适合在广西南宁及气候类似地区在避雨条件下进行一年两收栽培.

Grapevine is a popular cultivated fruit throughout the world and heat stress is one of the most serious threats to viticulture. However, transcriptional responses, such as molecular properties of photosynthesis and abscisic acid biosynthesis, metabolism and signal transduction pathway of grapevine to heat stress, are still poorly understood. In this study, RNA-seq was carried out for thermostabilized grapevine ‘Kyoho’ leaves. Results showed that 685 and 469 genes were commonly down-regulated and up-regulated at three sampling time-points. The light-dependent reactions of photosynthesis was significantly enriched in up-regulated DEGs at 1 hpt and down-regulated DEGs at R24 hpt. Heat stress impaired the photosynthetic capacity of grapevine leaves, and there was a significant positive relationship between photosynthesis and stomatal conductance at short-term post-heat stress treatment, but the inhibition of HS on Pn was non-stomata limitation for a longer period. Photosystem (PS)Ⅱ was more sensitive to heat stress than PSⅠ, and PsbP, as well as Psb28, played important roles in response to heat stress. The abscisic acid (ABA) content in heat-stress-treated Kyoho plants was higher than that in the control at 1 hpt, but less in heat-stress-treated plants at 4 and R24 hpt, which was regulated by numerous genes involved in the ABA biosynthesis and catabolism pathways. These results help to understand the influence of heat stress on photosynthesis and ABA biosynthesis, metabolism and signal transduction pathway.

为丰富广西葡萄栽培品种及调整产业结构,广西壮族自治区农业科学院葡萄与葡萄酒研究所引进了早熟葡萄品种春光、宝光,开展一年两收引种栽培试验,观察其在广西南宁地区的栽培性状、分析果实品质.结果表明:春光、宝光葡萄在广西南宁综合性状表现优良,为早熟品种,适合开展一年两收栽培,可进一步推广利用.

Powdery mildew is an economic threat for viticulture because it not only affects grape yield, but also causes a series of impacts on the qualities of fruit and wine, especially the flavors and various metabolites. Different grape varieties may have different levels of powdery mildew resistance/tolerance and their components of their metabolome are also various. In this study, two wine grape varieties, Guipu No.6 (GP6) and Marselan (Mar) with different levels of powdery mildew tolerance, were used to compare the quality differences in metabolism level by using the widely targeted metabolomics method. The results show that GP6 has a better powdery mildew leaf tolerance than Mar. A total of 774 metabolites were detected by using a UPLC-QQQ-MS-based metabolomics approach, and 57 differential metabolites were identified as key metabolites that were accumulated after infection with powdery mildew in GP6 and Mar, including phenolic acids, flavonoids, terpenoids, stilbenes, lipids, nucleotides and derivatives, lignans and coumarins, and quinones. This finding indicates that the defense mechanisms of grape fruit are mainly associated with phenylpropane-flavonoid metabolism. Specifically, stilbenes had greater variations after powdery mildew infection in GP6; while in Mar, the variations of flavonoids, especially kaempferol-3-O-glucuronide and luteolin-7-O-glucuronide, were more remarkable. The above results demonstrate that stilbenes may play a more important role than flavonoids in resisting powdery mildew infection in GP6's fruits, and the drastic variations of these phenolic compounds in different wine grapes after powdery mildew infection might also lead to quality difference in the flavors. This study can provide new insights into the understanding of the cause of powdery mildew tolerance in different grape varieties and the effects on the quality of wine grapes infected with the disease exerted by metabolism level.

在葡萄冬果发育期,采用田间自然鉴定的方法对广西避雨栽培的8个鲜食葡萄品种冬果发育期白粉病发病情况进行调查,同时结合同时期的气象条件进行分析,为适宜广西地区避雨栽培鲜食葡萄品种的选择、推广、白粉病防控以及种质资源创新提供依据.结果显示:8个供试鲜食葡萄品种冬果发育期最早于9月26日发现均感染白粉病,之后白粉病发病率和病情指数均随着冬果发育期发展而逐渐增加,在冬果发育期的前半段,不同遗传背景的鲜食葡萄品种之间白粉病发病率存在差异,在11月14日,病情指数差异最大,欧美杂种'夏黑'、'脆光'和欧亚种'沈农脆丰'3个品种均对葡萄白粉病表现感病,欧亚种'瑞都红玉'、'瑞都红玫'和欧美杂种'阳光乙女'、'蜜光'、'金光'5个供试鲜食葡萄品种均对白粉病高感.而到冬果发育后期,所有供试品种的白粉病发病率均接近100％;不同品种的病情指数尽管存在一定的差异,但最终所有品种病情指数均高于50.00％,高度感染白粉病.由于整个冬果发育期的气象条件适宜葡萄白粉病发生和流行,因此建议在生产管理上,8个鲜食葡萄品种在整个冬果发育期均需进行白粉病防控.

探索补光对阳光玫瑰(Shine Muscat)葡萄冬季果实(冬果)膨大期内源激素积累及果实品质的影响,以期为提高阳光玫瑰葡萄冬果栽培效益提供理论依据.以2016年定植的阳光玫瑰葡萄为试材,在葡萄冬果膨大期进行每天夜间延长光照6h处理,分别连续延长补光4周、5周和6周,每周采样一次.测定葡萄果实中玉米核苷(ZR)、吲哚乙酸(IAA)、赤霉素(GA3)和脱落酸(ABA)等4种内源激素含量,以及在果实成熟后测定果实品质的相关指标.结果 表明,葡萄冬果夜间补光可以提高果实IAA和GA3的含量,降低ABA的含量,其中补光6周处理的葡萄果实.IAA含量较高,比对照高83.4％,ABA含量比对照低62.30％.补光5周、6周处理对果实GA3含量提高较为明显.补光可以提高阳光玫瑰葡萄冬果单穗质量、单粒质量、可溶性固形物含量,降低可滴定酸含量,有效改善葡萄冬果的品质,增加冬果产量,明显增加经济效益.生产上建议在冬果膨大期进行补光处理,能增加冬果生长类内源激素的含量,增加产量,提高果实品质,其中补光时间以6周效果最好.

在广西南宁地区湿热气候条件下研究不同砧木对"阳光玫瑰"葡萄气灼病发病率、病情指数和果实品质的影响,以期筛选出气灼病发病率低、病情指数低、果实品质优良的砧木.以"SO4""5BB""1103P""夏黑""贝达"和"美人指"6种砧木嫁接的"阳光玫瑰"以及相同树龄的自根成年树(对照)为试材,调查和测定"阳光玫瑰"葡萄的气灼病发病率、病情指数、果实品质.结果表明,以"美人指""SO4""贝达""5BB"以及"1103P"为砧木能显著降低"阳光玫瑰"的气灼病发生率,除"夏黑"有着更高的发病率和病情指数外,其他均显著低于自根苗(p＜0.05).另外,各砧木均不同程度提升了"阳光玫瑰"的果实品质,其中以"5BB""夏黑"砧木最为显著.为提高"阳光玫瑰"果实品质和降低气灼病带来的损失,建议在南方湿热地区选用"5BB"作为"阳光玫瑰"的砧木.

2019-2020年连续2年对引进的无核鲜食葡萄品种"火洲黑玉""火洲红玉""火洲紫玉"开展引种观察试验.结果表明,3个品种均表现为生长势强,果肉硬脆等特点,"火洲黑玉"早熟,但极易裂果,不建议在南宁等地区推广种植,"火洲红玉""火洲紫玉"中熟,可在南宁及周边地区适当推广种植,适宜进行大棚避雨设施栽培.

试验以桂葡1号×赤霞珠及其343株杂交后代,桂葡1号×Lacrosse及其132株杂交后代作为试验材料,进行白粉抗性鉴定及遗传分析.结果表明:在桂葡1号与赤霞珠组合中,母本桂葡1号的病情指数为39.23,对白粉病表现为感病,父本赤霞珠病情指数为67.83,对白粉病表现为高感;343株F1代杂种群体,有77株高于父本赤霞珠的病情指数,有112株低于母本桂葡1号的病情指数.桂葡1号×Lacrosse组合,双亲对白粉病表现为感病,母本桂葡1号病情指数为39.23,父本病情指数为48.21;在132株F1代杂交种群体中,有42株高于父本Lacrosse的病情指数,有72株株低于母本桂葡1号的病情指数.在2个杂交后代群体中都分离出一定比例的高抗白粉病单株,为开展白粉病抗性葡萄新品种选育提供了材料.

[目的]探讨阳光玫瑰葡萄果实生长发育及品质对不同光质的响应,为提高果实品质提供参考依据.[方法]通过夜间对盛花后1周的阳光玫瑰葡萄每天进行6h补充红光、蓝光和白光处理,至果实转色完成后(共8周)停止补光,以不补光为对照,测定分析不同光质对果实生长发育期果实单粒重、横纵径、酒石酸、苹果酸及果实成熟采摘时可溶性固形物的影响.[结果]补光前期(补光1～4周)红光、蓝光和白光处理单粒重、纵径与对照无明显差异,补光后期(补光5～8周)各补光处理均能促进葡萄果实的单粒重、纵径、横径和可溶性固形物含量增加.与对照相比,红光处理6～8周对果粒横径有显著促进作用(P<0.05,下同),增幅为3.1％～8.1％,补光第7周的增幅最大;蓝光处理4～6周对果粒纵径有显著促进作用,增幅为3.8％～5.1％,最大差异出现在补光第6周.补光能增加酒石酸含量,红光在苹果酸代谢中起到积极作用,在果实转色后期能显著降低苹果酸含量5.1％～23.2％.补光对成熟期果实的糖酸比有促进作用,较对照显著增加7.5％～20.6％.成熟期果实生理和品质指标相关分析结果表明,补光后果实的单粒重、横径和纵径与果实酒石酸和苹果酸含量均呈极显著负相关(P<0.01).[结论]阳光玫瑰葡萄果实发育期进行夜间补充红光、蓝光和白光对提高果实单粒重、果粒纵径、横径、可溶性固形物含量和糖酸比有促进作用;红光在苹果酸代谢中起积极作用,在转色后期促进苹果酸降解.建议生产上在阳光玫瑰葡萄果实发育前期补充蓝光,促进果粒拉长,果实发育后期补充红光,增加可溶性固形物含量,促进苹果酸降解,提高果实品质.