Glyphosate-based herbicides are among the most widely used agricultural chemicals globally, and their widespread application presents risks to environmental health and aquatic ecosystems. Continuous glyphosate inputs disrupt phytoplankton communities, potentially triggering harmful algal blooms. This study examines the proliferation of microalgal species exposed to low glyphosate concentrations (0.05 mg/L) and various phosphorus sources, with a particular focus on C-P and C-O-P bond phosphonates, which have been insufficiently studied in previous research. We hypothesized that cyanobacteria might exhibit a competitive growth advantage over other algal species when exposed to C-P bond glyphosate, especially under phosphorus-limited conditions. In monoculture experiments, Microcystis aeruginosa and Peridinium umbonatum var. inaequale significantly increased their biomass when cultured with C-P bond phosphonates, whereas Scenedesmus bijuga failed to thrive under similar conditions. Peridinium umbonatum var. inaequale also displayed increased soluble protein content in response to glyphosate stress, indicating an adaptive stress response. In co-culture experiments, M. aeruginosa demonstrated greater tolerance to glyphosate than P. umbonatum var. inaequale, though biomass increases were not significantly correlated with soluble protein or APA. Sediment-water interface experiments revealed that glyphosate exposure significantly promoted cyanobacterial biomass, which was approximately five times greater than that of the inorganic phosphorus group. Notably, when cyanobacterial biomass exceeded 20% of the total, Cyanophyta replaced Chlorophyta as the dominant group, suggesting a potential competitive advantage under low-dose glyphosate exposure. These findings highlight that glyphosate may promote cyanobacterial dominance by altering phytoplankton community composition, potentially contributing to the increased frequency of harmful algal blooms in nutrient-limited aquatic environments.

Algal blooms of the dinoflagellate Karenia mikimotoi are seriously threats to the coastal ecosystems, particularly in developing countries. Many previous studies have focused on the effect of nutrients on algal blooms, but the accurate measurement of nutrients can be time-consuming, particularly in remote areas. Currently, the environmental drivers of K. mikimotoi proliferation are far from clearly understood in many coastal zones in the world. As a result of prevailing climate conditions and increasing eutrophication, the Pingtan Special Bay Area in China is prone to frequent red tide events. Based on field work conducted from 2013 to 2019, this study investigated the meteorological and hydrological variables influencing the proliferation of K. mikimotoi . Results showed that cell density of K. mikimotoi was significantly positively correlated with sea surface temperature (SST), air temperature (AT), and dissolved oxygen (DO) concentration, but negatively correlated with sea surface salinity (SSS) and air pressure (AP). A linear model between cell density and SST, SSS, AP, and DO was developed to assess and predict the risk of K. mikimotoi algal blooms ( R 2 = 0.810). Results are of practical significance for preventing and controlling the K. mikimotoi algal bloom in Southeast China. Furthermore, this proposed algal bloom prediction method based on the Linear Regression Model may potentially serve as a valuable reference for algal bloom risk forecasting and management.

为探究微纳米曝气对水库沉积物细菌及磷功能菌群落的影响,以三明市东牙溪水库为研究对象,通过Illumina高通量测序,分析了曝气前后沉积物细菌、聚磷菌与有机解磷菌的群落组成、结构及其与环境因子的相关性.结果表明,微纳米曝气对底部产生充氧作用,进而对沉积物微生物的群落造成一定影响,微纳米曝气后,变形菌门(Proteobacteria)的相对丰度上升了24.7％,厚壁菌门(Firmicutes)上升了8.3％,而绿弯菌门(Chloroflexi)下降了5.9％,酸杆菌门(Acidobacteria)下降了7.0％.曝气前,沉积物中聚磷菌的优势菌属包括Syntropphius(26.79％)、Anaeromyxobacter(14.6％)、Nitrospira(12.6％)和 Anaerolinea(11.3％);而曝气后,聚磷菌优势菌属则为Pseudomonas(65.1％)和Clostridium(22.9％).有机解磷菌的优势菌属在微纳米曝气前后同样发生了改变:在曝气前,优势菌属为Azospira、Mesorhizobium、Pseudomonas、Chielatococcus、Variovorax,分别占8.6％、5.4％、5.3％、5.2％、和5.2％;在曝气后,优势菌属则为 Azospira、Variovorax、Chelatococcus、Pseudomonas、Acidovorax,分别占11.3％、6.5％、6.1％、5.9％和5.8％.冗余分析结果表明,不管是聚磷菌还是有机解磷菌,其群落组成与沉积物表层的溶解氧(DO)均显著相关(P＜0.05).微纳米曝气工程的实施,对水库沉积物微生物的群落组成、结构有一定影响.

应用沉积物磷的标准测试程序(SMT)法对东张水库库区消落带土壤与沉积物的各形态磷进行测定,分析其分布特征.结果表明,消落带土壤中总磷(TP)含量在46.3～357.9 mg·kg-1,沉积物中TP含量在184.5～1348.8 mg·kg-1,沉积物TP含量平均值显著高于消落带样品TP含量平均值.消落带土壤与沉积物各种形态磷表现出不同的分布特点:沉积物中无机结合态磷(Fe/Al-P)占TP质量分数42.7％,明显高于消落带土壤无机结合态磷占比17.4％;而消落带土壤中有机磷(OP)占TP质量分数58.4％,远高于沉积物占比28.4％,消落带土壤与沉积物的OP和Fe/Al-P在TP中的含量均大于70.0％.相关分析结果显示,消落带土壤和沉积物中总有机碳(TOC)含量与各形态磷之间均呈极显著正相关(P<0.01),有机质(OM)含量与无机磷(IP)呈极显著正相关(P<0.01).

为研究水体浮游植物对不同浓度和不同形态磷的利用能力差异,采集福建九龙江江东库区冬季的水样,设置培养温度为20℃,分别进行原位水(TP=0.11mgL-1)、低浓度有机磷(单磷酸胞苷(5'-CMP)和无机磷(0.01mg·L-1)、高浓度的有机磷(单磷酸胞苷5'-CMP)和无机磷(1.00 mg·L-1)调控模拟实验.比较了不同藻群落结构起点,经磷调控培养后群落演替的情况,并对培养过程的藻细胞进行碱性磷酸酶荧光标记,观察碱性磷酸酶的分布情况.经过15 d的实验室培养,结果显示:原水组由隐藻(54.7％)、甲藻(28.0％)、绿藻(11.9％)演替为绿藻(67.7％)和硅藻(25.2％)为主,绿藻的增殖优势明显;低有机磷浓度模拟培养组演替为绿藻(63.7％)和硅藻(22.4％)为主,低浓度无机磷模拟培养组也是演替为绿藻(60.6％)和硅藻(28.3％)为主;高浓度有机磷模拟培养组浮游植物的结构从甲藻(52.8％)、隐藻(26.5％)、硅藻(7.4％)演替为甲藻(40.9％)和硅藻(32.9％)为主,高浓度无机磷组的结构演替为硅藻(52.1％)和绿藻(21.1％)为主;高浓度有机磷组培养过程中,绿藻门的栅藻(Scenedesmus)和楯形多甲藻不等变种(Peridinium umbonatum var.inaequale)细胞上观察到明显的碱性磷酸酶分布,表明两种藻都能利用单磷酸胞苷(5'-CMP),其中楯形多甲藻不等变种利用有机磷单磷酸胞苷能力更强.初春温度适宜且磷充足时,起点的藻群落结构和磷形态共同决定浮游植物群落的演替;当磷的浓度较低时,九龙江江东库区群落中绿藻门容易占据优势.本研究结果对九龙江藻类水华的防控具有现实意义.

The construction of cascade reservoirs increases eutrophication and exacerbates algal blooms and thus threatens water quality. Previous studies on the microalgae in reservoir have mainly focused on the spatio-temporal patterns of surface microalgae communities at the horizontal scale, while few studies have simultaneously considered the successions of microalgae in vertical profiles including the sediments and the effects of the nutrients release and microalgae in sediments on microalgae in upper waters. In this study, we investigated the effects of microalgae and physico-chemical parameters in waters and sediments on the successions of vertical microalgae communities in Xipi Reservoir, Southeast China. The seasonal variations in microalgae compositions decreased gradually from the surface water (the dominance of Cryptophyta and Chlorophyta in spring, Chlorophyta and Cyanophyta in summer, and relatively uniform in autumn and winter) to the sediment (the dominance of Bacillariophyta throughout the year), which was influenced by the variations of physico-chemical factors in different layers. The spatio-temporal variations in microalgae communities in waters was attributing to not only the heterogeneities of the stratification, and the physico-chemical factors such as water temperature, pH, and nutrient concentrations, especially for phosphorus in the water column, but also the combinations of phosphorus release and microalgae composition in sediments. Environmental changes would be especially problematic for microalgae groups such as Cryptophyta, Dinophyta and Chlorophyta that were sensitive to the changes of temperature and nutrients. Our results are helpful for an extensive understanding of the dynamics of microalgae communities in reservoir, and contribute to reservoir management for ensuring the safety of drinking water.

Microbial mineralization of organic phosphorus is an important component of marine phosphorus cycle. The research on organic phosphate-mineralizing bacteria (OPB) is helpful to reveal microbial driving mechanism of organic phosphorus mineralization in eutrophic sea area. The diversity and community characteristics of OPB were examined by Illumina high-throughput sequencing using the primer sets phoX in the sea area between Minjiang Estuary to Pingtan in April (spring) and July (summer) 2019. The results showed that the Shannon index of OPB in the surface seawater samples ranged from 3.21 to 7.91, and that the diversity at each station was greater in spring than that in summer. Shannon index of OPB in the sediment samples ranged from 2.04 to 8.70, which was greater in summer than that in spring. Shannon index of OPB in surface seawater of each station was higher than that of sediment in spring, while it was in adverse in summer. Nine phyla of OPB were detected in surface seawater, with Proteobacteria and Cyanobacteria being the most abundant. Tweleve phyla of OPB were detected in the sediments, with Proteobacteria and Bacteroidetes being the most dominant. OPB community composition at the genus level showed obvious spatio-temporal variation. Leisingera,Phaeobacter,Thalassococcus, and Pseudomonas were the major genera in the seawater in spring, while Synechococcus, Halioglobus, Roseovarius, Phaeo-bacter, Sulfitobacter, and Hyphomonas were the major genera detected in summer. Leisingera, Phaeobacter, Vibrio, and Sulfitobacter were major genera in the sediment in spring, while Azospirillum, Aminobacter, Sulfurifustis, Burkholderia, and Thiohalobacter were the major genera in summer. A large number of unclassified OPB were detected in both surface seawater and sediment. The redundancy analysis results showed that dissolved oxygen, water temperature, pH, dissolved inorganic nitrogen, NO2--N, and NO3--N had great influences on community distribution of OPB in the surface seawater. The abundant OPB in the surface seawater and sediment might play an important role in phosphorus cycle in this sea area.

为了探究含C—P键的草甘膦对多甲藻和微囊藻的增殖影响,以0.05 mg·L-1草甘膦为磷源开展实验室模拟实验.结果表明:单培养时,与起始生物量相比,多甲藻和微囊藻生物量分别增加了4.4倍和61.9倍;共培养时,多甲藻生物量占比从49.7％降至5.5％,而微囊藻生物量占比从50.3％升至94.5％.通过测定培养过程中两藻种生理生化指标的变化,推测微囊藻可能是利用草甘膦进而取得竞争优势,而多甲藻的增殖可能受低浓度草甘膦的刺激胁迫而增殖,水体环境中草甘膦的存在增加了蓝藻水华发生的可能.

One of the most challenging issues for developing countries in modern times is the care and management of clean, potable drinking water sources. Accordingly, this study singled out potential contributing factors to harmful algae blooms with a particular focus on phosphorus (P) release. The potential risks of P release for the drinking water from a lake in Fujian were assessed by investigating the spatial-temporal distribution of P, its exchange capacity, and its discharge capacity in sediment, including the community composition of phosphorus accumulating organisms (PAOs) and the phosphate’s initial migration effects on sediments. Different mixed materials, including soil aggregate distributions, sorbent, fractions, adsorption-desorption of P, and the community composition of PAOs were assessed. Total phosphorus (TP) content was measured at 24.4 ± 1.2 to 563.9 ± 38.2 mg/kg, but contents displayed some spatial differences. The dominant Ps found in the sample sediments were organic phosphorus and inorganic phosphorus associated with hydroxide Fe/Al-P, which accounted for 48.6% and 43.6%, respectively, of the TP content in the lake’s central waters. The TP concentration in vertical sediment ranged from 436.2 ± 21.3 to 602.9 ± 31.4 mg/kg. The TP spatio-temporal distribution inputs varied with rainfall (p < 0.05). P deposition occurred throughout most water bodies (p < 0.05), covering extensive areas and also decreasing at lower depths. Forty-four operational taxonomic unit (OTU) phosphorus-accumulating organism types from 11 phyla were detected in the sediment samples obtained from the Sanshiliujiao Lakes region. Proteobacteria also dominated compared to the organisms with the strongest PAOs. The diversity of PAOs in summer samples was significantly higher than that of the autumn samples. These findings provide a scientific foundation for determining the future discovery of the microbial mechanisms involved in the phosphorus metabolic cycle found in reservoir sediments. Various forms of phosphorus influenced the PAO diversity, especially Fe/Al-P. Thus, the abundance of PAOs in the sediment proved to be an essential component of the P cycle and may even play a key role in regional material circulation and in causing other environmental issues.

以闽江河口塔礁洲感潮淡水河岸野慈姑（Sagittaria trifolia Linn.）沼泽湿地为研究对象,通过连续一年每月脉冲式施加人造海水和Fe（OH） 3 溶液,研究了脉冲式盐水入侵和Fe（Ⅲ）浓度增强对河口感潮沼泽湿地土壤产甲烷菌和硫酸盐还原菌群落结构的影响.结果显示,单独Fe（Ⅲ）施加显著提高了土壤Fe（Ⅲ）含量,单独盐水施加提高了土壤电导率及间隙水SO 4 2- 、Cl - 和NH + 4 浓度,在Fe（Ⅲ）和盐水交互作用下间隙水NO - 3 浓度显著降低.土壤产甲烷菌主要包括甲烷微菌目（Methanomicrobiales）、八叠球菌目（Methanosarcinales）、甲烷杆菌目（Methanobacteriales）和Methanomassiliicoccales,硫酸盐还原菌主要有脱硫弧菌目（Desulfovibrionales）、脱硫杆菌目（Desulfobacterales）、互营杆菌目（Syntrophobacterales）和梭菌目（Clostridiales）.无论是脉冲式盐水入侵、Fe（Ⅲ）浓度增强或是两者交互作用对于土壤产甲烷菌和硫酸盐还原菌多样性及群落结构的影响均不显著.与目水平相比,硫酸盐还原菌在属水平上对于各添加处理的响应更为明显,其中盐水+Fe（Ⅲ）处理下Desulfatibacillum和Desulfovirga的相对丰度明显增加.RDA分析表明,土壤电导率对产甲烷菌群落结构的影响最为显著,Fe（Ⅲ）是影响硫酸盐还原菌的重要环境因子,且Desulfatibacillum、Desulfobulbus、Desulfovirga等菌属对环境因子的响应较为敏感.研究表明,脉冲式盐水入侵及三价铁浓度增加对于我国东南沿海河口感潮淡水沼泽湿地土壤产甲烷菌和硫酸盐还原菌无显著影响.

为了探究不同藻竞争利用不同形态磷对浮游植物群落结构影响,分别以楯形多甲藻不等变种(Peridinium umbonatum var.inaequale)和铜绿微囊藻(Microcystis aeruginosa)进行PM4A板多磷源单培养实验,并向原位水样添加其两藻种进行5'-单磷酸腺苷(5'-AMP)和磷酸氢二钾(K2HPO4)增殖模拟实验.结果表明:不同的藻种对不同形态磷的竞争利用具有选择性,楯形多甲藻不等变种和铜绿微囊藻分别能够利用溶解性有机磷(DOP)35种和25种,两种藻能够较好的利用氨基酸和核苷酸.当以楯形多甲藻不等变种调控起始生物量占比(绿藻:甲藻:硅藻:蓝藻为38％:26％:20％:7％),无论以有机磷还是无机磷为磷源,甲藻的竞争优势明显,生物量占比达37.11％～50.19％;当以铜绿微囊藻调控起始生物量占比(绿藻:蓝藻:硅藻:甲藻为38％:29％:20％:4％),蓝藻竞争优势明显,生物量占比达52.25％～53.44％.在温度和光照等环境条件一定的情况下,磷源形态和藻类起始生物量结构共同影响浮游植物群落结构演替.

采用PM4 A多磷源板(共59种磷源)单种培养东海原甲藻(Prorocentrum dong-haiense),研究不同形态磷对其增殖的影响.结果表明:东海原甲藻可以利用47种磷源(其中42种有机磷,5种无机磷),对有机态磷的利用效率高于无机磷,主要对含有C-O-P酯键的有机磷吸收利用效果更好,其中利用效果较好的有机磷包括磷酸乙醇酸、D-甘露糖-1-磷酸和磷酸烯醇式丙酮酸.表明有机磷可能是影响东海原甲藻生长的重要磷源.本文探讨有机磷在东海原甲藻繁殖过程中所起到的作用,为研究东海原甲藻赤潮的发生规律和应对措施提供依据,具有现实意义.

Polyphosphate accummulating organisms (PAOs) play an important role in the phosphorus metabolic cycling in the sediment of reservoir. We assessed the diversity and community structure of PAOs in the sediments by T-RFLP and clone sequencing which targeted ppk1 gene at the hearts of three reservoirs (Jiulongjiangxipi reservoir, Sanshiliujiao lake reservoir and Dongyaxi reservoir) in Fujian Province. The results showed that the diversity of PAOs varied among different reservoirs, though not statistically significant. The diversity of PAOs in the Sanshiliujiao lake reservoir was highest (Shannon index H=2.89±0.03, Simpson index D=0.06±0.01). The community structure of the PAOs in the Sanshiliujiao lake reservoir was most complicated, consistent with the results of the T-RFLP. The differences of dominant PAOs genera in three reservoirs were distinct, mainly concentrated in the Proteobacteria, Actinobacteria and Acidobacteria. The percentage of those three phylum accounted for 74.5%, 85.0% and 75.0%, respectively, of the total PAOs. The dominant groups in each reservoir sediment were Anaeromyxobacter and Solibacter. Various forms of phosphorus had certain influence on the diversity of PAOs. There were significantly correlation between Fe/Al-P and PAOs diversity and community structure. The dominant genus in the three reservoirs, Anaeromyxobacter, was positively correlated with all forms of phosphorus and significantly correlated with insoluble phosphorus such as OP and Ca-P, while Solibacter was negatively correlated with all forms of phosphorus. The results suggested that PAOs had important impacts on the phosphorus cycle of sediment in eutrophicatied reservoirs.

为了提升学生环境工程微生物学实验的设计与操作技能,实现在实践教学中培养创新人才的目标,以“沉积物功能菌群的分子表征”为例,通过沉积物DNA的提取、功能基因的扩增、琼脂糖凝胶电泳、扩增产物的回收及纯化、克隆文库的构建、克隆测序以及基因多样性分析等系列实验,把环境微生物学实验中涉及的基础实验有机组合成一个内容相关联的创新型大实验,进一步强化环境生物技术中微生物学、分子生物学和生态学三大知识体系的有机联系,提高了环境工程微生物学实验教学的创新性和综合性,促进了学生参与科技创新的积极性,提升了实践教学水平,取得了良好的教学效果.

磷是浮游植物生长的必要元素,也是调控水体富营养化的重要因子之一.在无机磷缺乏的水域,能够有效利用有机磷的藻类有可能成为浮游植物群落的优势种.为了研究有机磷对浮游植物群落结构演替的影响,分别从3个水库采集表层水样,西陂水库(硅藻70.3％,甲藻19.1％,绿藻9.3％)、山美水库(硅藻31.3％,甲藻59.2％,绿藻3.5％)、三十六脚湖(硅藻43.1％,甲藻50.5％,绿藻5.0％),选用有机磷单磷酸腺苷(Adenosine monophosphate,AMP)作为磷源进行实验室模拟实验,并与无机磷(NaH2PO4·2H2O)作为磷源实验组做同步对比,探讨有机磷源条件下浮游植物竞争及群落结构演替规律.结果显示:AMP培养20 d后,西陂水库、山美水库和三十六脚湖的浮游植物生物量分别增加了6.5倍,2.1倍和1.9倍,浮游植物群落结构都演替为以甲藻门和绿藻门为优势藻,所占比例分别为81.8％和16.7％、55.2％和33.5％、73.2％和24.3％,其中西陂水库甲藻门拟多甲藻对有机磷AMP的利用能力强,其所占比例达到81.8％.表明有机磷AMP能有效地促进甲藻门拟多甲藻属的增殖,进而改变水体浮游植物群落结构.

采用末端限制性片段长度多态性(Terminal restriction fragment length polymorphism,T-RFLP)技术结合克隆文库构建,分析福建省平潭岛三十六角湖水库夏冬季特定样点沉积物聚磷菌(PAO)多样性及群落组成.结果显示,三十六脚湖沉积物中夏季聚磷菌的多样性明显高于冬季,夏季优势聚磷菌末端限制性片段(Terminal restriction fragment,T-RF)集中在200-300 bp,而冬季聚磷菌T-RF片段集中在150-200 bp.克隆测序和系统发育分析表明,三十六脚湖水库沉积物样品中检测到优势的聚磷菌类群为变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和酸杆菌门(Acidobacteria).夏季丰度大于10％优势的菌属为厌氧粘细菌属(Anaeromyxobacter)、固氮螺菌属(Azospirillum)、聚磷小月菌(Microlunatus phosphovorus)和伯克氏菌属(Burkholderia),而冬季丰度大于14％优势的菌属主要有厌氧粘细菌属、甲基杆菌属(Methylobacterium)、Solibacter属、固氮螺菌属和聚磷小月菌.上述结果表明三十六脚湖沉积物中聚磷菌多样性及群落组成均呈现出一定的季节变化特征,沉积物各形态磷对聚磷菌多样性有一定影响,且与铁铝结合态磷相关性最显著,这可为今后揭示水库沉积物磷代谢循环的微生物学机制提供科学基础.

To reveal the effects of different phosphorus forms on the growth and succession of phytoplankton community,we investigated the phytoplankton community change at sediment-water interface in Xipi Reservoir,Jiulong River,with different phosphorus sources,as inorganic phosphate (IP,NaH2PO4 · 2H20),organic phosphorus (adenosine monophosphate,AMP),sediment phosphorus extracted by SMT methods (SMT-TP),fresh sediment (Sediment) and original water (OW).The phytoplankton abundance in Xipi Reservoir is 7.2 × 104 cells· L-1,dominated by Diatom (49.16％),Chlorophyta (32.40％) and Cyanobacteria (17.32％).Our results show a significant higher growth rate of phytoplankton in IP and AMP treatments (N ∶P =20∶1),where the dominant phyla in phytoplankton community changed to Cyanobacteria and Chlorophyta,accounting for 76.67％ and 23.34％ of total population,respectively.The phytoplankton community in AMP treatment was dominated by Chlorophyta (92.51％).In treatments of low phosphorus (N ∶P-25∶1),OW (N ∶ P =17∶ 1) and SMT-TP (N ∶ P =10∶1),the dominant phyla in phytoplankton community were Diatoms,Chlorophyta and Cyanobacteria,consistent with the results in IP treatment.However in Sediment treatment (N ∶ P =10∶1,contain both inorganic and organic phosphorus),the dominant phyla changed to Cyanobacteria,accounting for 71.80％ of total population.The results indicate the significant impacts of phosphorus forms on phytoplankton community structure.In all the IP,AMP and SMT-TP treatments,the diversity of phytoplankton community declined and Diatom gradually became rare phylum.On the contrast,the diversity of phytoplankton community remained stable in Sediment and OW treatments,and Diatom was still the major phylum.It implied that natural sediment can maintain and regulate phytoplankton community structure.The dominancy of Cyanobacteria in Sediment treatment suggested that the release of phosphorus from sediment has an important contribution to the cyanobacteria bloom.

沉积物既是营养盐的储存库,也是藻类的休眠场所.本研究以九龙江北溪江东水库为研究对象,于2011年4月、6月、9月、12月采集表层沉积物,对其营养盐含量、表层沉积物微藻分布及丰度进行分析.结果表明,沉积物大部分为粉砂质粘壤土,该区域沉积物总碳含量为9140.2～17760.4 mg·kg-1,总氮含量为950.0～1600.4 mg·kg-1,总磷含量为483.7～856.2 mg·kg-1.库区表层沉积物微藻细胞丰度范围在7.41×105 ～2.17×106 cells·g-1底泥(以千重计),主要为硅藻门和绿藻门,分别占微藻比例的45.52％ ～ 86.42％和5.96％～35.55％.沉积物微藻总丰度与上覆水体浮游植物总丰度及隐藻、裸藻丰度呈显著负相关,反映了沉积物微藻对上覆水体浮游植物的影响.沉积物中蓝藻、隐藻、甲藻孢囊丰度分别与水温呈显著负相关,分别与溶解氧呈显著正相关,因而冬季沉积物中有相对高的微藻丰度.沉积物微藻丰度与含水率、粉粒分别呈显著性正相关,反映了沉积物微藻沿着水流方向的积累.