Solid-phase denitrification using biodegradable polymers such as polycaprolactone (PCL) is increasingly proposed as a sustainable alternative to conventional soluble carbon dosing. However, reported kinetic and stoichiometric parameters for PCL-based systems are often confounded by mass-transfer and dissolution limitations, hindering accurate determination of biological kinetics. In this study, the dissolution step was explicitly decoupled from microbial growth by presolubilizing PCL prior to use, enabling the direct experimental determination of biomass yield (Y) and maximum specific growth rate (μmax) under strictly anoxic batch conditions. Methanol was investigated in parallel as a benchmark soluble carbon source. Yield assays conducted over 12 independent replicates produced statistically similar yields of 0.35 ± 0.08 and 0.38 ± 0.08 g COD per g COD for methanol and PCL, respectively, indicating comparable stoichiometric efficiency for biomass synthesis. Dynamic nitrate depletion profiles were resolved through high-frequency batch testing and fitted using SUMO process modeling. The resulting μmax values were 1.3 day-1 for methanol and 2.1 day-1 for presolubilized PCL, indicating that when carbon availability is not limited by dissolution, under the tested decoupled batch conditions, PCL-derived substrates can support higher growth rates than conventional methanol. Long-term acclimation tests showed lower net biomass accumulation with PCL, attributable to its slower carbon release rather than to microbial capacity limits. These results provide the first direct determination of true biological kinetic parameters for PCL-based denitrification, independent of polymer hydrolysis effects. The findings demonstrate that the apparent kinetic constraints previously reported for solid PCL systems primarily reflect physicochemical mass-transfer limitations and not biological capacity. This work establishes design-ready kinetic parameters for integrating biodegradable polymers into predictive denitrification models, supporting the rational implementation of biodegradable polymeric carbon sources in sustainable nitrogen removal processes.

Nitrate pollution in aquatic environments poses significant environmental and public health issues, mostly due to industrial activities and agricultural runoff. Biological denitrification, the favored method for removing nitrates, typically needs an external carbon source to support microbial processes. Traditional electron donors like methanol, ethanol, and acetate are effective but introduce economic, environmental, and operational challenges such as cost variability, flammability hazards, and excessive residual organic material. Recently, solid-phase carbon sources—like biodegradable polymers and organic agricultural waste—have shown promise as alternatives because they allow for controlled carbon release, improved safety, and enhanced long-term sustainability. This review systematically examines the performance of solid-phase carbon in wastewater denitrification by analyzing peer-reviewed studies and experimental data. The findings suggest that solid-phase carbon sources, including polycaprolactone (PCL) and polyhydroxyalkanoates (PHA), offer stable and extended carbon release, ensuring consistent denitrification effectiveness. Nonetheless, challenges remain, including optimizing biofilm development, balancing carbon availability, and reducing operational costs. Furthermore, the review emphasizes the potential for integrating machine learning in process optimization and highlights the need for more research to enhance the economic viability of these materials. The findings confirm the practicality of solid-phase carbon sources for extensive wastewater treatment and their capability to sustainably address nitrate contamination.

Understanding biofilm thickness and the half-saturation coefficient (Ks) is essential for optimizing biofilm systems to remove micropollutants from water effectively. The study aimed to explore how biofilm thickness and Ks impact the biodegradation of the selected micropollutants (salicylic acid, acetaminophen, and ibuprofen), considering their slow and fast biodegradation nature. Findings revealed that the thinnest biofilms achieved faster biodegradation, with 100 % salicylic acid removal in 8 h in contrast to >13 h for the thickest biofilms. Similar patterns were observed for removal of both acetaminophen and ibuprofen. The study also found that biodegradation by thin and thick biofilms is correlated with the Ks, implying that the Ks value could potentially be reduced by decreasing biofilm thickness. Moreover, 16S rRNA gene sequencing highlighted diverse microbial communities in biofilms, with distinct dominant bacterial genera associated with each micropollutant. For instance, Salicylic acid degrading biofilms had dominant genera like Rhodoferax (21.7 %), Polaromonas (21.4 %), and Undibacterium (16.6 %). Meanwhile, acetaminophen degrading biofilm had a high proportion of Pseudomonas (62.9 %). Ibuprofen degrading biofilms were distinct, with Sediminibacterium being the primary genus at 28.5 %.

The frequent design challenge for existing water resource recovery facilities targets the accommodation of an similar to 50% load increase within the existing infrastructure and footprint. Off-loading this organic load at the top-end of the plant and redirection toward the digesters has proven the most efficient way of process intensification. The Triple A settler is an "activated primary treatment," stands for alternating activated adsorption, and can be retrofitted into existing rectangular or circular (mostly) primary tanks at a hydraulic retention time of 2 h and a sludge retention time of about 0.5 days. Several technology implementations demonstrate flexible designs adjusting to existing tank geometries and depths of 2.5 to 5.0 m. Different implementation scales from dry-weather flow rates ranging from 0.1 to 10 mgd show generic applicability of the functional principles at any scale: Biosorption, bioflocculation, and assimilation provide the key added value in pretreatment efficiencies of similar to 60/25/33 in %COD/%N/%P removal compared with application of pure physics in primary settling with typical 33%/9%/11% removal, respectively. Practitioners Points center dot Triple A is a hybrid form of A-stage and contact stabilizer for advanced primary treatment. center dot Besides COD and TSS, also, P and N can be removed via Triple A. center dot Triple A can be retrofitted in existing rectangular or circular tanks. center dot This high-rate process does not worsen the conditions for enhanced biological phosphorus removal. center dot Energy efficiency, capacity increase, and operational benefits are the main goals of Triple A.

Sub-disciplines of the water sector are fractured into specialty domains. So the underlying modeling tools for flow and water quality are designed based on a pre-determined set of equations. However, many problems involve interactions between multiple domains in an integrated 'one water' approach. The opportunity to integrate the different sets of equations is fundamental to the shift towards evaluating environmental issues more holistically. This paper describes an extensible modeling framework for defining and adding user-defined model components using "plugins". The plugin approach allows the available model components and the processes to adapt to the user's specific needs and objectives. The focus of this paper is to describe the philosophy of developing the open extendable framework and to present the data structure used to describe model components, their properties, and the equations used for computing state and derived variables. In the end, four examples of the framework's multi-domain capability are provided.

There have been significant advances in the use of biological and physical selectors for the intensification of continuously flowing biological wastewater treatment (WWT) processes. Biological selection allows for the development of large biological aggregates (e.g., mobile biofilm, aerobic granules, and densified biological flocs). Physical selection controls the solids residence times of large biological aggregates and ordinary biological flocs, and is usually accomplished using screens or hydrocyclones. Large biological aggregates can facilitate different biological transformations in a single reactor and enhance liquid and solids separation. Continuous-flow WWT processes incorporating biological and physical selectors offer benefits that can include reduced footprint, lower costs, and improved WWT process performance. Thus, it is expected that both interest in and application of these processes will increase significantly in the future. This review provides a comprehensive summary of biological and physical selectors and their design and operation.

Currently, RCRA requires hazardous waste incinerators to be evaluated using principal organic hazardous constituents (POHCs) to calculate destruction removal efficiencies (DREs). POHCs are compounds listed in Appendix VIII of 40 CFR Part 261.3. To receive an operating permit, a hazardous waste incinerator must demonstrate it is capable of destroying or removing at least 99.99 percent of a processed substance. This demonstration process is known as a Trial Burn. Sulfur hexafluoride (SF6), as a surrogate POHC to demonstrate incineration performance, has been proposed as an economical and safe strategy to facilitate this demonstration.

High-rate activated sludge (HRAS) systems suffer from high variability of effluent quality, clarifier performance, and carbon capture. This study proposed a novel control approach using bioflocculation boundaries for wasting control strategy to enhance effluent quality and stability while still meeting carbon capture goals. The bioflocculation boundaries were developed based on the oxygen uptake rate (OUR) ratio between contactor and stabilizer (feast/famine) in a high-rate contact stabilization (CS) system and this OUR ratio was used to manipulate the wasting setpoint. Increased oxidation of carbon or decreased wasting was applied when OUR ratio was <0.52 or >0.95 to overcome bioflocculation limitation and maintain effluent quality. When no bioflocculation limitations (OUR ratio within 0.52-0.95) were detected, carbon capture was maximized. The proposed control concept was shown for a fully automated OUR-based control system as well as for a simplified version based on direct waste flow control. For both cases, significant improvements in effluent suspended solids level and stability (<50-mg TSS/L), solids capture over the clarifier (>90%), and COD capture (median of 32%) were achieved. This study shows how one can overcome the process instability of current HRAS systems and provide a path to achieve more reliable outcomes. PRACTITIONER POINTS: Online bioflocculation boundaries (upper and lower limit) were defined by the OUR ratio between contactor and stabilizer (feast/famine). To maintain effluent quality, carbon oxidation was minimized when bioflocculation was not limited (0.52-0.95 OUR ratio) and increased otherwise. A fully automated control concept was piloted, also a more simplified semiautomated option was proposed. Wasting control strategies with bioflocculation boundaries improved effluent quality while meeting carbon capture goals. Bioflocculation boundaries are easily applied to current wasting control schemes applied to HRAS systems (i.e., MLSS, SRT, and OUR controls).

Sidestream partial nitritation and deammonification (pN/A) of high-strength ammonia wastewater is a well-established technology. Its expansion to the mainstream is, however mainly impeded by poor retention of anaerobic ammonia oxidizing bacteria (AnAOB), insufficient repression of nitrite oxidizing bacteria (NOB) and difficult control of soluble chemical oxygen demand and nitrite levels. At the municipal wastewater treatment plant in Strass (Austria) the microbial consortium was exhaustively monitored at full-scale over one and a half year with regular transfer of sidestream DEMON® biomass and further retention and enrichment of granular anammox biomass via hydrocyclone operation. Routine process parameters were surveyed and the response and evolution of the microbiota was followed by molecular tools, ex-situ activity tests and further, AnAOB quantification through particle tracking and heme measurement. After eight months of operation, the first anaerobic, simultaneous depletion of ammonia and nitrite was observed ex-situ, together with a direction to higher nitrite generation (68% of total NOx-N) as compared to nitrate under aerobic conditions. Our dissolved oxygen (DO) scheme allowed for transient anoxic conditions and had a strong influence on nitrite levels and the NOB community, where Nitrobacter eventually dominated Nitrospira. The establishment of a minor but stable AnAOB biomass was accompanied by the rise of Chloroflexi and distinct emergence of Chlorobi, a trend not seen in the sidestream system. Interestingly, the most pronounced switch in the microbial community and noticeable NOB repression occurred during unfavorable conditions, i.e. the cold winter season and high organic load. Further abatement of NOB was achieved through bioaugmentation of aerobic ammonia oxidizing bacteria (AerAOB) from the sidestream-DEMON® tank. Performance of the sidestream pN/A was not impaired by this operational scheme and the average volumetric nitrogen removal rate of the mainstream even doubled in the second half of the monitoring campaign. We conclude that a combination of both, regular sidestream-DEMON® biomass transfer and granular SRT increase via hydrocyclone operation was crucial for AnAOB establishment within the mainstream.

The James R. Dolorio Water Reclamation Facility in Pueblo, Colorado, uses AvN aeration controls to lower aeration energy while promoting carbon-efficient nutrient removal and hydrocyclone-based wasting to achieve SVI improvements and process intensification. The results from the full-scale installation showed that hydrocyclone-based wasting helped improve settling characteristics by reducing the SVI from 200 ± 52 mL/g to 83 ± 22 mL/g within weeks of operation. PAO and nitrifiers were preferentially retained in dense flocs and granules, while lighter heterotrophic and filamentous organisms were preferentially wasted, thus uncoupling the SRT of these two fractions relative to overall SRT. The SRT of dense aggregates was estimated at 14.4 ± 3.4 days and for lighter flocs at 7.1 ± 2.3 days. The use of AvN control with continuous low DO conditions resulted in low DO conditions (< 0.4 mgO2/L) reducing air demands by 50% while providing excellent nitrogen (effluent TIN < 11 mg/L) and TP removal (effluent TP < 1 mg/L) at low primary effluent COD/N ratio of 6.0. The presence of comammox was demonstrated through molecular analysis, while ex-situ batch tests revealed the presence of DPAO, which could have attributed to the energy and carbon-efficient biological nutrient removal.

Retrofitting conventional denitrification filters into partial denitrification-anammox (PdNA)- or anammox (AnAOB)-based filters will reduce the needs for external carbon addition. The success of AnAOB-based filters depends on anammox growth and retention within such filters. Studies have overlooked the importance of media selection and its impact on AnAOB capacity, head loss progression dynamics, and shear conditions applied onto the AnAOB biofilm. The objective of this study was to evaluate viable media types (10 types) that can enhance AnAOB rates for efficient nitrogen removal in filters. Given the higher backwash requirement and lower AnAOB capacity of the conventionally used sand, expanded clay (3-5 mm) was recommended for AnAOB-based filters in this study. Owing to its surface characteristics, expanded clay had higher AnAOB activity (304- vs. 104-g NH4+-N/m(2)/day) and higher AnAOB retention (43% more) than sand. Increasing the iron content of expanded clay to 37% resulted in an increase in zeta potential, which led to 56% more anammox capacity compared to expanded clay with 7% iron content. This work provides insight into the importance of media types in the growth and retention of AnAOB in filters, and this knowledge could be used as basis in the development of PdNA filters. Practitioner Points Expanded clay showed the lowest head loss buildup and most likely will result in longer runtime for full-scale PdNA applications The highest AnAOB rates were achieved in expanded clay types and sand compared with smaller media typically used in biofiltration Expanded clay resulted in better AnAOB retention under shear, whereas sand could not withstand shear and required more frequent backwashing Expanded clay iron coating enhanced AnAOB enrichment and retention, most likely due to increased surface roughness and/or positive charge

This study demonstrates the potential of an innovative anaerobic treatment technology for municipal biosolids (IntensiCarb), which relies on vacuum evaporation to decouple solids and hydraulic retention times (SRT and HRT). We present proof-of-concept experiments using primary sludge and thickened waste activated sludge (50-50 v/v mixture) as feed for fermentation and carbon upgrading with the IntensiCarb unit. IntensiCarb fully decoupled the HRT and SRT in continuously stirred anaerobic reactors (CSAR) to achieve two intensification factors, that is, 1.3 and 2, while keeping the SRT constant at 3 days (including in the control fermenter). The intensified CSARs were compared to a conventional control system to determine the yields of particulate hydrolysis, VFA production, and nitrogen partitioning between fermentate and condensate. The intensified CSAR operating at an intensification factor 2 achieved a 65% improvement in particulate solubilization. Almost 50% of total ammonia was extracted without pH adjustment, while carbon was retained in the fermentate. Based on these results, the IntensiCarb technology allows water resource recovery facilities to achieve a high degree of plant-wide intensification while partitioning nutrients into different streams and thickening solids. PRACTITIONER POINTS: The IntensiCarb reactor can decouple hydraulic (HRT) and solids (SRT) retention times in anaerobic systems while also increasing particulate hydrolysis and overall plant capacity. Using vacuum as driving force of the IntensiCarb technology, the system could achieve thickening, digestion, and partial dewatering in the same unit-thus eliminating the complexity of multi-stage biosolids treatment lines. The ability to partition nutrients between particulate, fermentate, and condensate assigns to the IntensiCarb unit a key role in recovery strategies for value-added products such as nitrogen, phosphorus, and carbon, which can be recovered separately and independently.

Full-scale demonstration of activated sludge conversion into a granule-floc hybrid process was implemented in Dijon (France) water resource recovery facility (WRRF). Biomass densification was achieved based on external gravimetric selection using hydrocyclones within continuous-flow anaerobic-anoxic-oxic (A(2)O) biological nutrient removal (BNR) bioreactor. The goal was to optimize settleability of biological sludge by lowering and stabilizing sludge volume index (SVI) to improve process robustness and resiliency. Process proved to stabilize operation and to uncouple the total solids residence time (SRT) between floc and granule morphologies. The densified biomass initially produced stable SVI < 100 ml/g for a period of 4 months and thereafter a steady state year-round SVI below 50 ml/g, including the winter period during which the control train SVI expansion >200 ml/g. The densified biomass successfully broke the vicious cycle of interannual bulking. Form and function interrelationship is proposed for the densified biomass (hybrid floc-granule). The concept of biological architecture is proposed as the purposeful control of granule and floc proportions, with a proposed "form factor" ratio of 1:2 granule to floc, that produce a "SRT uncoupling function factor" ratio of 4:1 granule to floc, further resulting in very stable settling and effluent functionalities. Practitioner Points Controlling granule-floc proportions allows for sludge volume index (SVI) operational adjustment, which further allows for increased clarified design accuracy. One-third aggregates dramatically improved settling characteristics: 20% and 35% of AGS ensures SVIs below 100 and 50 ml/g, respectively. Densified biomass enables new SRT and clarifier flux rates approaches for engineering and operation practices: Doubling typical surface loading rates from 6.0-8.5 to 15-20 kg m(-2) h(-1) and surface overflow rates from 0.6-0.8 to 1.5-2.4 m/h SRT uncoupling of 1:4 is achieved between floc and granule, enabling specific niche environment for fast and slow growing organisms.

Improved settleability has become an essential feature of new wastewater treatment innovations. To accelerate adoption of such new technologies, improved clarifier models are needed to help with designing and predicting improvement in settleability. In general, the level of mathematics of settling clarifier models has gone far beyond the level of existing experimental methods available to support these models. To date, even for simple one-dimensional (1D) clarifier models, no experimental method has been described for flocculent settling coefficient (rp). As a consequence, rp cannot be considered as a sludge characteristic and is used as a calibration parameter to achieve observed effluent quality. In this study, we focused on the development of an empirical function based on a simple and practical experimental approach for the calculation of the rp value from sludge characteristics. This approach provided a similar approach as currently taken for hindered settling coefficient calculations (Veslind equation) and allowed for the model to predict effluent quality, thus increasing the power of the 1D model. The threshold of flocculation (TOF), which describes the collision efficiency of particles, directly correlated with the effluent quality of the five tested activated sludge systems and was selected as experimental method. The proposed empirical function between TOF and rp was validated for four years of validating data with five different sludge types operated under different operational conditions and configurations. The good effluent quality prediction with this approach brings us one step closer in making the clarification models more predictive towards effluent quality and clarifier performance.

This study examined the biodegradation of two pharmaceuticals-acetaminophen, and ibuprofen, and one natural organic surrogate-salicylic acid, by bacteria seeded from backwash water collected from a full-scale biofiltration plant. The degradation was studied in the presence of oxygen. Complete removal of salicylic acid was observed in 27-66 h depending on the seasonality of the collected backwash water, while 90-92% acetaminophen removal was observed in more than 225 h. Ibuprofen demonstrated poor removal efficiencies with only 50% biodegradation after 230 h. Adenosine tri phosphate (ATP) in the reactor was found to be linked with the biodegradation rate. ATP was found to be correlated with oxygen uptake rate (OUR). ATP also had a correlation with each of extracellular polymeric substances (EPS), protein and polysaccharides. These results highlight the potential for increasing the biodegradation rates to achieve enhanced contaminant removal.

Primary sludge fermentate, a concentrated hydrolyzed wastewater carbon, was evaluated for use as an alternative carbon source for mainstream partial denitrification-anammox (PdNA) in a suspended growth activated sludge process in terms of partial denitrification (PdN) efficiency, PdNA nitrogen removal contributions, and final effluent quality. Fermenter operation at a 2-day sludge retention time (SRT) resulted in the maximum achievable yield of 0.14 +/- 0.05 g sCOD/g VSS without release of excessive ammonia and phosphorus to the system. Based on the results of batch experiments, fermentate addition led to PdN efficiency of 93 +/- 14%, which was similar to acetate at a nitrate residual of 2-3 mg N/L. In the pilot-scale mainstream deammonification reactor, PdN efficiency using fermentate was 49 +/- 24%, which was lower than acetate (66 +/- 24% during acetate period I and 70 +/- 21% during acetate period II), most probably due to lower nitrate and ammonium kinetics in the PdN zone. Methanol cost-saving potential for the application of PdNA as the main short-cut nitrogen pathway was estimated to be 30% to 55% depending on the PdN efficiency achieved. Practitioner points Primary sludge fermentate was evaluated as an alternative carbon source for mainstream partial denitrification-anammox (PdNA). Fermenter operated at a 1 to 2 day SRT resulted in the maximum achievable yield without the release of excessive ammonia and phosphorus to the system. Although 93% partial denitrification efficiency was achieved with fermentate in batch experiments, around 49% PdN efficiency was achieved in pilot studies. Application of PdNA with fermentate can result in significant methanol cost savings.