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Major Research Accomplishments
• We have produced recombinant buffalo chymosin in bacterial and yeast expression systems for their use as milk clotting enzyme required for cheese production
• Isolation and purification protocol of buffalo and goat native chymosin established
• 3D structure and biochemical characterization of buffalo chymosin, BRP39 and MGP40 have been determined by x-ray crystallography
• Lactoferrins from various animal species have been purified and characterized
• Cloned and characterized oviduct specific glycoprotein from buffaloes and goat
• Proteome map of cattle and buffalo mammary epithelial cells during lactation
• Isolation and expression analysis of MGP-40 and IGFBP-5 in lactation animals.
Guidance to student:
• We have produced recombinant buffalo chymosin in bacterial and yeast expression systems for their use as milk clotting enzyme required for cheese production
• Isolation and purification protocol of buffalo and goat native chymosin established
• 3D structure and biochemical characterization of buffalo chymosin, BRP39 and MGP40 have been determined by x-ray crystallography
• Lactoferrins from various animal species have been purified and characterized
• Cloned and characterized oviduct specific glycoprotein from buffaloes and goat
• Proteome map of cattle and buffalo mammary epithelial cells during lactation
• Isolation and expression analysis of MGP-40 and IGFBP-5 in lactation animals.
Guidance to student:
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