Department of Anesthesiology and Pain Medicine University of Washington/Department of Chemistry, University of Washington
My research group focuses on the development of new methods in and applications of metabolite profiling using a combination of mass spectrometry and NMR platforms which provide a broad based approach for biomarker discovery and systems biology research. We have worked closely with a large number of scientific and clinical collaborators and biostatisticians on various metabolomics projects such as the identification of cancer biomarkers for early detection, recurrence monitoring and therapy prediction. We are also involved in studies of diabetes and heart diseases as well as nutrition based disease prevention and aging. Our group has a strong interest in expanding the understanding of disease risk factors using metabolite profiling approaches. We have extensive experience in the quantitative determination of metabolite concentrations in a variety of samples including serum/plasma, urine, cells and tissue extracts. In conjunction with the Northwest Metabolomics Research Center that we established several years ago, we have developed a large number of assays and approaches for metabolite profiling. We utilize a range of platforms including targeted LC-MS/MS for broad based and quantitative profiling and targeted pathway assays, LC-MS for global aqueous profiling and lipidomics, and GC-MS and NMR global profiling methods that provide excellent coverage of the metabolome. We also perform isotope tracer analysis using GC-MS and NMR based approaches. We have significant experience and expertise in the use of a number of multivariate statistical methods as well as metabolic pathway analysis tools. My group has experience running large metabolomics sample sets that incorporate a number of quality control measures to ensure reliability of large datasets.