The increasing emergence of bacterial strains resistant to antibiotics is far outpacing our ability to develop novel therapies to treat these infections. Further, the methodologies used to both diagnose infection and predict appropriate courses of treatment are antiquated. Thus, novel ways of thinking about diagnosing and treating infectious diseases must be developed. By merging the powerful fields of chemical biology and bacterial genetics/genomics, we hope to provide insight into possible new paradigms for addressing infectious diseases. The goal of the Hung lab is three fold: 1) to understand mechanisms of bacterial pathogenesis that might better predict both host and pathogen genes that are required for survival of the pathogen in vivo and that might ultimately suggest new ways to intervene based on disrupting the pathogen-host interaction; 2) identifying bacterial functions essential for in vivo survival and persistence (survival in the face of antibiotic therapy), and small molecule tools with which to probe the biology of these functions in vivo; and 3) identifying transcriptional responses to antibiotic stress and developing new tools to both rapidly diagnose a pathogen and determine antibiotic susceptibility based on these responses. Examples of approaches taken include high-throughput forward chemical screens, genomic characterization including single cell analysis, classical forward and reverse genetics, and multiple in vivo models to address these areas, which are described in more detail below.