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For many years I studied insect cuticular proteins (CPs) as molecular markers of metamorphic stage., now I am interested in the precise role CPs play in constructing insects. We began by annotating the CP genes of Anopheles gambiae, the major vector of malaria. Unexpectedly, we learned that Anopheles devotes over 2% of its protein coding genes to CPs. Shot-gun proteomics analyses established that 82% of the genes we called CPs actually code for proteins found in the cuticle. Some of these are present in the genome as clusters of highly similar genes. Once we had identified the Anopheles CP genes, we turned to learning when and where they are expressed. A comprehensive analysis with quantitative real-time RT-PCR revealed that most mRNAs for CPs are found in brief periods immediately before or after a molt. In situ hybridization revealed where transcripts for specific CPs were located. We learned where in the cuticle secreted proteins are located using secondary antibodies labeled with colloidal gold on EM sections. All of the adult structures we studied have dozens of CPs. So far we have evidence for only 4 CPs being restricted to a single structure, many are present and abundant in all structures.
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论文共 84 篇作者统计合作学者相似作者
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,Geoffrey M Attardo, P P Abila, J Auma,Aaron A Baumann,Joshua B Benoit,Corey Brelsfoard, Jose M C Ribeiro,James A Cotton,Daphne Q D Pham,Alistair C Darby,Jan Van Den Abbeele,
SCIENCEno. 6182 (2014): 380-386
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