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Neuronal Signal Transduction
Our research focuses on synaptic plasticity, the ability of synapses to change their connection strength. This process is thought to underlie learning and memory. Cascades of biochemical reactions in dendritic spines, tiny (~0.1 femtoliter) postsynaptic compartments emanating from dendritic surfaces, trigger diverse forms of synaptic plasticity. The Yasuda lab aims to elucidate some of the operation principles of such signaling networks in dendritic spines using various optical techniques.
First, we have been developing techniques to image activity of various proteins in single dendritic spines using 2-photon fluorescence lifetime imaging microscopy (2pFLIM) in combination with new biosensors extensively optimized for 2pFLIM. Using this technique, we have succeeded in imaging signaling proteins, CaMKII, HRas, RhoA and Cdc42, in single dendritic spines undergoing synaptic potentiation. Our results indicate that signaling activity is subjected to a complicated spatiotemporal regulation: some signals are compartmentalized in single synapses, while others spread to affect a short stretch (micrometers) of dendritic segments including multiple synapses. This rich spatiotemporal regulation plays an essential role in coordinating cellular events in different dendritic micro-compartments. Further, Ca2+ elevation in a synapse is relayed in several different stages to induce long-term plasticity: First, Ca2+ signals in the synapse are integrated by a signaling protein CaMKII, of which activity decays over ~10 s. Then, Ras, Cdc42 and RhoA are activated by CaMKII, and relay this transient signal into signals lasting 10-30 min.
We have been developing many more sensors to reveal the precise temporal sequences of signaling events in different microcompartments. In addition, we have been developing tools to activate and inactivate signaling proteins in spines optically. Recently we have developed a photo-inducible CaMKII inhibitor, and demonstrated that 60 s of CaMKII activation is required to activate downstream signaling. By monitoring and manipulating activity of signaling proteins with high spatiotemporal resolution, we hopefully disentangle the complicated signaling networks and understand the signaling mechanisms underlying synaptic plasticity and ultimately learning and memory.
研究兴趣
论文共 166 篇作者统计合作学者相似作者
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bioRxiv : the preprint server for biology (2024)
biorxiv(2024)
Jun Yao Wu, Su-Ji Cho, Katherine Descant,Peter H. Li,Alexander Shapson-Coe,Michal Januszewski,Daniel R. Berger, Cailyn Meyer, Cristine Casingal, Ariba Huda, Jiaqi Liu, Tina Ghashghaei,
NEURONno. 1 (2024): 41-55.e3
Isabel Espadas, Jenna L. Wingfield,Yoshihisa Nakahata, Kaushik Chanda, Eddie Grinman, Ilika Ghosh, Karl E. Bauer, Bindu Raveendra,Michael A. Kiebler,Ryohei Yasuda,Vidhya Rangaraju,Sathyanarayanan Puthanveettil
Nature Communicationsno. 1 (2024): 1-24
Science advancesno. 31 (2023)
引用2浏览0WOSSCIENCE引用
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bioRxiv : the preprint server for biology (2023)
Research square (2023)
Anant Jain,Yoshihisa Nakahata,Tetsuya Watabe, Polina Rusina, Kelly South, Kengo Adachi,Long Yan,Noriko Simorowski,Hiro Furukawa,Ryohei Yasuda
bioRxiv : the preprint server for biology (2023)
引用1浏览0WOS引用
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Shouvik Majumder, Koichi Hirokawa,Zidan Yang, Ronald Paletzki,Charles R Gerfen,Lorenzo Fontolan,Sandro Romani,Anant Jain,Ryohei Yasuda,Hidehiko K Inagaki
bioRxiv : the preprint server for biology (2023)
Lesley A Colgan,Paula Parra-Bueno,Heather L Holman,Xun Tu,Anant Jain, Mariah F Calubag, Jaime A Misler, Chancellor Gary, Goksu Oz, Irena Suponitsky-Kroyter, Elwy Okaz,Ryohei Yasuda
biorxiv(2023)
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