Neoplasms.

2006317 The molecular mechanism of PML in inducing apoptosis of bladder cancer cell line. LI Lei, et al. Urol, 1st Hosp Xi′an Jiaotong Univ Shanxi 710061. Dept Natl Med J Chin 2005;85(25);1766-1769. Objective:Study the effect of inducing expression of PML in the apoptosis and the molecular mechanism of bladder cancer. Methods:PMEP4/PML inducible expression vector was transfected into bladder cancer UM-UC-2 cells by lipofectamine2000 system. The positive clone cells were selected by 300μg/ml hygromycin B and confirmed by laser confocal imaging system. Then, using the in vitro DNA ladder apoptotic assay and Western blot, the effect of inducing expression of PML on apoptosis and its molecular mechanism of bladder cancer cell was studied. Results:Comparing with the vector control group, PML specific nuclear speckle significantly increased in the PMEP4/PML bladder cells. DNA ladder assay demonstrated bladder cancer cell expressing PML occurred apoptosis while the control vector cells were not influenced. Overexpression of PML could reduce Survivin expression and upregulate caspase3 and cleaved PARP protein expression.