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Pcr-Based Subtractive Hybridization And Differences In Gene Content Among Strains Of Helicobacter Pylori

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, no. 22 (1998): 13108-13113

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academic.microsoft.com pubmed.ncbi.nlm.nih.gov

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Genes that are characteristic of only certain strains of a bacterial species can be of great biologic interest. Here we describe a PCR-based subtractive hybridization method for efficiently detecting such DNAs and apply it to the gastric pathogen Helicobacter pylori, Eighteen DNAs specific to a monkey-colonizing strain (J166) were obtaine...更多

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