Leukotriene D4 Stimulates the Migration but Not Proliferation of Endothelial Cells Mediated by the Cysteinyl Leukotriene CysLT1 Receptor via the Extracellular Signal-Regulated Kinase Pathway
Journal of Pharmacological Sciences(2009)
摘要
The actions of cysteinyl leukotrienes (CysLTs) are mediated by activating CysLT receptors, CysLT1, and CysLT2. The CysLT1 receptor mediates vascular responses to CysLTs; however, its effect on the proliferation and migration of endothelial cells is not clarified. To determine this effect, we observed proliferation and migration in EA.hy926 cells, a human endothelial cell line, and the involvement of activation of mitogen-activated protein kinases (MAPKs). We found that LTD4 did not affect the proliferation, but significantly stimulated the migration of endothelial cells. LTD4 also induced the phosphorylation of extracellular signal-regulated kinase (ERK) 1/2, but not those of p38 or JNK. The LTD4-induced migration and ERK1/2 phosphorylation were blocked by the CysLT1-receptor antagonist montelukast and the dual antagonist Bay u9773, but not by the CysLT2-receptor antagonist Bay cysLT2; the migration was also inhibited by the ERK1/2 inhibitor U0126. Our findings indicate that LTD4 stimulates the CysLT1 receptor–mediated migration of endothelial cells; this may be regulated by the ERK1/2 pathway.
更多查看译文
关键词
cysteinyl leukotriene receptor 1 (CysLT1),endothelial cell,proliferation,migration,extracellular signal-regulated kinase (ERK) 1 /2
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要