A highly efficient procedure for site-specific mutagenesis of full-length plasmids using Vent DNA polymerase.
Genome research, no. 4 (1995): 404-407
Careful titration of Vent polymerase activity allows efficient amplification of full-length plasmids (12 kb). The high processivity and fidelity of this enzyme made oligonucleotide-directed site-specific mutagenesis of plasmids a straight-forward process. Using only two primers, a mutagenic and a complementary, single-base mutants of reco...更多
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