An effective method for the in situ synthesis of DNA-CPG conjugates using chemical ligation technology as tools for SNP analysis.

Bioorganic & Medicinal Chemistry Letters（2007）

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摘要

A new method for the SNP analysis by using a chemical ligation (CL) technique on CPG plates with high coupling efficiency is reported.

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关键词

Chemical ligation,Gene detection,SNP,N-unprotected DNA synthesis,2-Thiothymidine,Base recognition

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