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Formation of azole-resistant Candida albicans by mutation of sterol 14-demethylase P450.

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, no. 5 (1999): 1163-1169

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enzymeamino acidpolymorphismwild typecatalytic activity

Abstract

The sterol 14-demethylase P450 (CYP51) of a fluconazole-resistant isolate of Candida albicans, DUMC136, showed reduced susceptibility to this azole but with little change in its catalytic activity. Twelve nucleotide substitutions, resulting in four amino acid changes, were identified in the DUMC136 CYP51 gene in comparison with a reported...More

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