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Induction Of Stable Rna Interference In Mammalian Cells

GENE THERAPY, no. 6 (2006): 503-508

Cited: 61|Views26

WOS NATURE

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pubmed.ncbi.nlm.nih.gov academic.microsoft.com

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RNA interference micro-RNAs short hairpin RNAs lentiviral vectors

Abstract

Over the last years, RNA interference (RNAi) has become a widely used technique that permits the knock-down, and hence functional analysis, of individual genes in vertebrate cells. However, the high failure rate of the RNA molecules used in RNAi experiments continues to be a problem. In this paper, I describe a set of design criteria, exp...More

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Bryan R Cullen

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