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Let-7 Microrna-Mediated Mrna Deadenylation And Translational Repression In A Mammalian Cell-Free System

GENES & DEVELOPMENT, no. 15 (2007): 1857-1862

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MicroRNAs (miRNAs) are incorporated into miRNP complexes and regulate protein expression post-transcriptionally through binding to 3'-untranslated regions of target mRNAs. Here we describe a recapitulation of let-7 miRNA-mediated translational repression in a cell-free system, which was established with extracts prepared from HEK293F cell...更多

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