, sp. nov., a novel bacterium isolated from oil-polluted site produces extracellular surface activity
Systematic and Applied Microbiology(2005)
摘要
During screening for biosurfactant-producing bacteria, a strain designated J36T was isolated from oil-polluted site near Kaohsiung city located in southern Taiwan. Cells of this organism were Gram-negative rods motile by means of a single polar flagellum. Strain J36T grew well in complex media under optimum conditions of 35°C and pH 7. The extracellular products of the strain expressed emulsification activity. During cultivation on olive oil as the sole carbon and energy source, the culture supernatant of strain J36T reduced surface tension of the medium from 68 to 32.6dyne/cm. The 16S rRNA gene sequence analysis indicates that strain J36T is a member of Xanthomonas group within the γ-Proteobacteria. The organism belongs to the genus Pseudoxanthomonas and represents a novel species within this genus according to phylogenetic analysis of 16S rDNA sequences, DNA–DNA similarity data, whole-cell protein analysis, physiological and biochemical characteristics, as well as fatty acid compositions. The predominant cellular fatty acids of strain J36T were 15:0 iso (about 26%), 17:1 iso ω9c (about 25%), and 15:0 anteiso (about 10%). Its DNA base ratio was 60.1mol% G+C. We propose to classify strain J36T (=BCRC 17375T=LMG 22530T) as Pseudoxanthomonas kaohsiungensis sp. nov.
更多查看译文
关键词
Pseudoxanthomonas kaohsiungensis,Xanthomonas,Biosurfactant,Taxonomy
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络