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In-Vitro Activation Of Urease Apoprotein And Role Of Urea As A Chaperone Required For Nickel Metallocenter Assembly

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, no. 8 (1994): 3233-3237

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ACCESSORY PROTEIN KLEBSIELLA-AEROGENES BI-NICKEL CENTER

Abstract

The formation of active urease in Klebsiella aerogenes requires the presence of three structural genes for the apoprotein (ureA, ureB, and ureC), as well as four accessory genes (ureD, ureE, ureF, and ureG) that are involved in functional assembly of the metallocenter in this nickel-containing enzyme. Slow and partial activation of urease...More

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I S Park

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M B Carr

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R P Hausinger

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