An efficient method for in vitro labeling proteins to high specific radioactivity using 3H-NaBH4 in dimethylformamide
Analytical Biochemistry(1981)
摘要
A method for labeling proteins or insoluble protein matrices to high specific radioactivity using 3H-NaBH4 in anhydrous dimethylformamide without the presence of formaldehyde or other aldehydes and ketones is presented. Chemical analysis of the labeled proteins suggests that the labeling mechanism involves methylation of lysyl residues. The procedure is simple and the labeled protein products retain their size and charge characteristics.
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关键词
chemical analysis,enzyme activity
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