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Stable Isotope Labeling By Amino Acids In Cell Culture, Silac, As A Simple And Accurate Approach To Expression Proteomics

MOLECULAR & CELLULAR PROTEOMICS, no. 5 (2002): 376-386

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Quantitative proteomics has traditionally been performed by two-dimensional gel electrophoresis, but recently, mass spectrometric methods based on stable isotope quantitation have shown great promise for the simultaneous and automated identification and quantitation of complex protein mixtures. Here we describe a method, termed SILAC, for...更多

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