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In vitro reactivation of spindle elongation in fission yeast nuc2 mutant cells.

JOURNAL OF CELL BIOLOGY, no. 2 (1990): 417-425

Cited: 67|Views30

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temperaturemicrotubulesphenotypemitosismutation

Abstract

To investigate the mechanisms of spindle elongation and chromosome separation in the fission yeast Schizosaccharomyces pombe, we have developed an in vitro assay using a temperature-sensitive mutant strain, nuc2. At the restrictive temperature, nuc2 cells are arrested at a metaphase-like stage with short spindles and condensed chromosomes...More

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