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Validation of a quantitative method for real time PCR kinetics.
Biochemical and Biophysical Research Communications, no. 2 (2002): 347-353
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摘要
Real time RT-PCR is the most sensitive method for quantitation of gene expression levels. The accuracy can be dependent on the mathematical model on which the quantitative methods are based. The generally accepted mathematical model assumes that amplification efficiencies are equal at the exponential phase of the reactions for the same am...更多
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