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Overexpression Of A Glutamate Receptor (Glur2) Ligand Binding Domain In Escherichia Coli: Application Of A Novel Protein Folding Screen

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, no. 25 (1997): 13431-13436

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AMPA receptor in vitro folding ligand-gated ion channel inclusion bodies fractional factorial folding screen

Abstract

Expression of the S1S2 ligand binding domain [Kuusinen, A., Arvola, M. & Keinanen, K. (1995) EMBO J 14, 6327-6332] of the rat alpha-amino-3-hydroxy-5-methylisoxazole-3-propionic acid-selective glutamate receptor GluR2 in Escherichia coli under control of a T7 promoter leads to production of >100 mg/liter of histidine-tagged S1S2 protein (...More

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Guo-qiang Chen (陈国强)

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Eric Gouaux

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