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2,4,6-trinitrotoluene reduction by an Fe-only hydrogenase in Clostridium acetobutylicum.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, no. 3 (2003): 1542-1547

Cited: 59|Views25
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Abstract

The role of hydrogenase on the reduction of 2,4,6-trinitrotoluene (TNT) in Clostridium acetobutylicum was evaluated. An Fe-only hydrogenase was isolated and identified by using TNT reduction activity as the selection basis. The formation of hydroxylamino intermediates by the purified enzyme corresponded to expected products for this react...More

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