Ceramide Generation Is Sufficient To Account For The Inhibition Of The Insulin-Stimulated Pkb Pathway In C2c12 Skeletal Muscle Cells Pretreated With Palmitate

We have employed C2C12 myotubes to investigate lipid inhibition of insulin-stimulated signal transduction and glucose metabolism. Cells were preincubated for 18 h in the absence or presence of free fatty acids (FFAs) and stimulated with insulin, and the effects on glycogen synthesis and signaling intermediates were determined. While the u...更多