HPLC study of the binding of sulfinpyrazone to serum albumin

Hummel-Dreyer method was used to study the binding of sulfinpyrazone to bovine serum albumin. The technique involves our newly developed internal calibration method with the elution pattern consisting of two positive peaks and with the integrator to evaluate the area of second peak. From the area of the second peak the numerical value ofLb (the amount of drug bound to protein) was calculated. Sctachard-Rosenthal plot was used to estimate the binding parametersni (the number of binding sites) andki (the binding constants). The results are compared with those obtained by equilibrium dialysis.