[Cloning and Bio-Informatics Analysis of the CsCrSP from Clonorchis Sinensis].

OBJECTIVE:To screen cDNA library from Clonorchis sinensis for gene identification, and to clone and construct library of secreted recombinant proteins.METHODS:cDNA library from Clonorchis sinensis was screened for identifying new genes by Blastn protocol, the sequence of which was further analyzed by Motifscan and NCBI Conserved Domain Search protocol.RESULTS:The secreted proteins (Pcs004f03) were identified, with a 689 bp DNA sequence (187aa), and a theoretical molecular weight of Mr 21100. It is predicted that the gene CsCrSP contains one N-glycosylation site, three N-myristoylation sites, three casein kinase II phosphorylation sites, one cAMP-and cGMP-dependent protein kinase phosphorylation site and protein kinase C phosphorylation site, a signal peptide at N-terminals and SCP-like extracellular protein domain.CONCLUSION:The CsCrSP gene is a secretory cysteine-rich protein with a SCP-like extracellular protein domain.