Palladium Catalyzed Synthesis of Ca2+ Indicators with Aryl Bithiophene and Terthiophene Fluorophores
Tetrahedron(2006)
摘要
Five new fluorescent indicators for Ca2+ were synthesized using the Stille reaction. They all consist of the tricarboxylate chelator APTRA (o-aminophenol-N,N,O-triacetic acid) linked to a (substituted) bithiophene or terthiophene fluorophore. The dissociation constants Kd measured via fluorimetric titrations at 21°C in 100mM KCl buffered solution, pH 7.05, for the Ca2+ complexes with the new probes are in the range between 10 and 40μM.
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关键词
fluorescent indicator,thiophene,Stille reaction,Ca2+
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