8-Hydroxydeoxyguanosine induces senescence-like changes in KG-1, human acute myelocytic leukemia cell line

8-Hydroxydeoxyguanosine (oh 8 dG) treatment induced senescence-like changes in KG-1 cells, a human acute myelocytic leukemia cell line. The oh 8 dG-treated cells stained positive for senescence associated β-galactosidase (SA-β-galactosidase) and had enlarged cell shape, both of which are senescence indexes. The oh 8 dG-treated cells were also cell growth inhibited and arrested at G 1 in the cell cycle. The accumulation of cdk (cyclin dependent kinase) inhibitors, such as p16, p21, and p27, also implies that cellular senescence was induced in oh 8 dG-treated cells. However, these changes were not accompanied by cell differentiation or telomerase activity. Taken together, we conclude that oh 8 dG treatment of KG-1 cells induces cellular senescence.