AI帮你理解科学

AI 生成解读视频

AI抽取解析论文重点内容自动生成视频


pub
生成解读视频

AI 溯源

AI解析本论文相关学术脉络


Master Reading Tree
生成 溯源树

AI 精读

AI抽取本论文的概要总结


微博一下
The average force values obtained for puncturing the outer membrane of flying fish egg cells and salmon fish egg cells was 1.6057mN and 2.2694mN respectively

Force Feedback Interface for Cell Injection

WHC, pp.391-400, (2005)

引用57|浏览35
EI WOS
下载 PDF 全文
引用
微博一下

摘要

Manual pronuclei injection and intracytoplasmic sperm injection (ICSI) requires long training and has low success rates primarily due to poor control over the injection force. Consequently, there is a need for quantification of forces during biological cell injection and for an automated cell injection system, which can provide force feed...更多

代码

数据

0
简介
  • Bio-surgery on individual cells, ordered cell arrays or aggregates of cells will be the platform for the generation of genetic manipulation.
  • To achieve bio-surgery reliably and accurately, operators must have accurate haptic (“feel”) and visual feedback from the cell as intracellular injection is conducted.
  • A haptic and visual feedback system can be used to manipulate an individual cell or array of cells to standardize outcomes of cellular.
  • Surgical procedures
  • Further advancement of this technology can be reliable and accurate gene injection at specific target sites within the cell or in the nucleus
重点内容
  • Bio-surgery on individual cells, ordered cell arrays or aggregates of cells will be the platform for the generation of genetic manipulation
  • This paper describes a force feedback interface for reflecting forces to the user during cell membrane puncturing tasks
  • The successful implementation and calibration of the force sensor has been presented in detail
  • The average force values obtained for puncturing the outer membrane of flying fish egg cells and salmon fish egg cells was 1.6057mN and 2.2694mN respectively
  • During all membrane puncture tasks the user was clearly able to discern when the membrane was punctured through a rapid drop in the force felt through the PHANToM
方法
  • PVDF (Polyvinylidene fluoride) piezoelectric polymer film is used to develop the force sensor for measuring the cell injection forces.
  • The glass micropipette is integrated to the PVDF film (Thickness: 28μm, Model: LDT1-028K of MSI, Inc.) with the help of a connector as shown in the Figure 2.
  • This setup allows the easy removal and replacement of the micropipette if the tip of it gets damaged during micromanipulation (5μm ID).
  • The following parameters will be used in the analysis: W: width of PVDF Film h: thickness of PVDF film L: length of PVDF Film A: surface Area (L * W) a: cross-sectional area (W * h) Q(t) : charge produced (PVDF Film) I(t): Current produced (PVDF film) V(t) : voltage across the PVDF Film RP : resistance of PVDF Film CP : Capacitance of the PVDF Film F(t) : Contact force
结果
  • To test the force feedback interface for cell injection, the authors performed several cell injections on two different types of egg cells, namely, salmon fish egg cell and flying fish egg cell.
  • The experimental setup consisted of the PVDF film, nanomanipulator, micropipette (Tip ID: 5μm) inserted into the hollow connector, and the PHANToM haptic interface device.
  • A plastic micropipette holds the
结论
  • This paper describes a force feedback interface for reflecting forces to the user during cell membrane puncturing tasks.
  • The average force values obtained for puncturing the outer membrane of flying fish egg cells and salmon fish egg cells was 1.6057mN and 2.2694mN respectively.
  • During all membrane puncture tasks the user was clearly able to discern when the membrane was punctured through a rapid drop in the force felt through the PHANToM.
  • The work presented in this paper cannot be directly applied to puncture smaller cells in the range of 50-100μm diameter.
  • Human factors studies comparing automated vs. manual cell injection to improve the cell viability after injection will be the natural future direction of this research
基金
  • * We acknowledge the support of National Science Foundation grant: CAREER IIS-
引用论文
  • P. Kallio and J. Kuncova, "Manipulation of Living Biological Cells: Challenges in Automation," presented at Workshop on Microrobotics for Biomanipulation at IROS Conference, Las Vegas, 2003.
    Google ScholarFindings
  • T. Fukuda, M. Fujiyoshi, F. Arai, and H. Automation, Sacramento, USA, 1991.
    Google ScholarFindings
  • Y. Kimura and R. Yanagimachi, Biology of Reproduction, vol. 52, pp. 709-720, 1995.
    Google ScholarLocate open access versionFindings
  • T. Tanikawa and T. Arai, "Development of a Robotics and Automation, vol. 15, pp. 152 - 162, 1999.
    Google ScholarLocate open access versionFindings
  • K. K. Tan and S. C. Ng, "Computer-Controlled Education Journal, pp. 249 - 256, 2001.
    Google ScholarLocate open access versionFindings
  • K. K. Tan, S. C. Ng, and Y. Xie, "Optimal Intra-Cytoplasmic Sperm Injection with a iezo Congress on Intelligent Control and Automation, Shanghai, China, 2002.
    Google ScholarFindings
  • P. Gao and S.-M. Swei, "A Six-Degree-of- Piezoelectric Translator," Nanotechnology, vol.
    Google ScholarLocate open access versionFindings
  • 10, pp. 447 - 452., 1999.
    Google ScholarFindings
  • [8] S. Guo, H. Zhang, and S. Hata, "Complex
    Google ScholarFindings
  • [9] P. Kallio, M. Lind, Q. Zhou, and H. N. Koivo, "A Conference on Robotics and Automation, Leuven, Bel-gium, 1998.
    Google ScholarFindings
  • [10] K.-M. Lee and S. D.K, "Kinematic Analysis of a Conference on Robotics and Auto-mation, Raleigh, USA, 1987.
    Google ScholarFindings
  • [11] Y. Ohya, T. Arai, Y. Mae, K. Inoue, and T. Robots and Systems, Kyonjyu, Korea, 1999.
    Google ScholarLocate open access versionFindings
  • [12] A. Codourey, R. Siegwart, W. Zesch, and R. Büchi, "A Robot System for Automated Handling in Micro-World," presented at Intelligent Robots and Systems, Pittsburgh, USA, 1995.
    Google ScholarFindings
  • [13] M. Goldfarb and J. E. Speich, "Design of a International Conference on Robotics and Automation, Albuquerque, New Mexico, USA, 1997.
    Google ScholarFindings
  • [14] H. Morishita and Y. Hatamura, "Development of Ultra Micro Manipulator System Under Stereo SEM Observation," presented at IEEE International Conference on Intelligent Robots and Systems, Yokohama, Japan, 1993.
    Google ScholarLocate open access versionFindings
  • [15] A. Bergander, W. Driesen, T. Varidel, and J.-M. Breguet, "Development of Miniature Manipulators for Applications in Biology and Nanotechnologies," presented at Workshop on Microrobotics for Biomanipulation at IROS Conference, Las Vegas, 2003.
    Google ScholarFindings
  • [16] P. Ge and J. M., "Tracking Control of a Piezoceramic Actuator," IEEE Trans-actions on Control Systems Technology, vol. 4, 1996.
    Google ScholarLocate open access versionFindings
  • [17] D. Croft, G. Shedd, and S. Devasia, "Creep, Hysteresis, and Vibration Com-pensation for Piezoactuators: Atomic Force Microscopy Application," presented at American Control Conference, Chicago, Illinois, 2000.
    Google ScholarLocate open access versionFindings
  • [18] G. S. Choi, H.-S. Kim, and G. H. Choi, "A Study on Position Control of Piezo-electric Actuators," presented at IEEE International Symposium on Industrial Electronics, Guima-raes,Portugal, 1997.
    Google ScholarLocate open access versionFindings
  • [19] C. V. Newcomb and I. Flinn, "Improving the Linearity of Piezoelectric Ceramic Actuators," Electronics Letters, vol. 18, 1982.
    Google ScholarLocate open access versionFindings
  • [20] P. Ronkanen, P. Kallio, and H. Koivo, "Current Control of Piezoelectric Actuators with Power Loss Compensation," presented at IEEE/RSJ International Conference on Intelligent Robots and Systems, Lausanne, Switzerland, 2002.
    Google ScholarLocate open access versionFindings
  • [21] P. Ronkanen, P. Kallio, Q. Zhou, and H. Koivo, "Current Control of Piezoelectric Actuators with Environmental Compensation," presented at Micro.tec 2003 the 2 nd VDE World Microtechnologies Congress, Munich, Germany, 2003.
    Google ScholarLocate open access versionFindings
  • [22] R. H. Comstock, "Charge Control of Piezoelectric Actuators to Reduce Hysteresis Effects." USA, 1981.
    Google ScholarFindings
  • [23] R. Perez, J. Agnus, J.-M. Breguet, N. Chaillet, H. Bleuler, and R. Clavel, "Characterisation and Control of a 1DOF Monolithic Piezoactuator (MPA)," presented at SPIE: Microrobotics and Microassembly III, Boston, USA, 2001.
    Google ScholarLocate open access versionFindings
  • [24] K. Furutani, M. Urushibata, and N. Mohri, "Improvement of Control Method for Piezoelectric Actuator by Combining Induced Charge Feedback with Inverse Transfer Function Compensation," presented at IEEE International Conference on Robotics and Automation, Leuven, Belgium, 1998.
    Google ScholarLocate open access versionFindings
  • [25] T. Arai, A. Suzuki, Y. Kato, Y. Mae, K. Inoue, and T. Tanikawa, "Automated Calibration for Intelligent Robots and Systems, Lausanne, Switzerland, 2002.
    Google ScholarFindings
  • [26] A. Kawaji, F. Arai, and T. Fukuda, "Calibration for Contact Type of Micro-manipulation, presented at Proceedings of the 1999 IEEE/RSJ
    Google ScholarLocate open access versionFindings
  • International Conference on Intelligent Robots and Systems, Kyongju, Korea, 1999.
    Google ScholarFindings
  • [27] G. Li and N. Xi, "Calibration of a Robots and Systems, Lausanne, Switzerland, 2002.
    Google ScholarFindings
  • [28] F. Arai, K. Morishima, T. Kasugai, and T. and Systems, pp. 1300-1305, 1997.
    Google ScholarLocate open access versionFindings
  • [29] F. Arai, A. Kawaji, P. Luangjarmekorn, T. Automation, pp. 604-609, 2001.
    Google ScholarLocate open access versionFindings
  • [30] M. W. Berns, "Laser Scissors and Tweezers," in Scientific American, 1998, pp. 52 -57.
    Google ScholarLocate open access versionFindings
  • [31] F. Arai, M. Ogawa, T. Mizuno, T. Fukuda, K. Intelligent Robots and Systems, Kyonjyu, Korea, 1999.
    Google ScholarLocate open access versionFindings
  • [32] M. Nishioka, S. Katsura, K. Hirano, and A. Transactions on Industry Applications, vol. 33, pp. 1381-1388, 1997.
    Google ScholarLocate open access versionFindings
  • [33] M. Pesce and M. De Felici, "Purification of Biology, vol. 170, pp. 722 -725, 1995.
    Google ScholarLocate open access versionFindings
  • [34] T. Kozuka, T. Tuziuti, H. Mitome, T. Fukuda, and F. Arai, "Two-dimensional Acoustic Science, Nagoya, Japan, 1998.
    Google ScholarFindings
  • [35] Y. Zhou, B. J. Nelson, and B. Vikramaditya, Micromanipulation," International Conference on Robotics & Automation, pp. 1220-1225, 1998.
    Google ScholarLocate open access versionFindings
  • [36] Y. Sun, B. J. Nelson, D. P. Potasek, and E. Enikov, "A bulk microfabricated multi-axis capacitive cellular force sensor using transverse comb drives," Journal of Micromechanics and Microengineering, vol. 12, pp. 832-840, 2002.
    Google ScholarLocate open access versionFindings
  • [37] B. Vikramaditya and B. Nelson, "Visually Guided Microassembly Using Optical Microscopes and Active Vision Techniques," presented at IEEE International Conference on Robotics and Automation, Albuquerque, New Mexico, USA, 1997.
    Google ScholarLocate open access versionFindings
  • [38] J. T. Feddema and R. W. Simon, "CAD-Driven Microassembly and Visual Servoing," presented at IEEE International Conference on Robotics and Automation, Leuven, Belgium, 1998.
    Google ScholarLocate open access versionFindings
  • [39] J. Korpinen, P. Kallio, and J. Viitanen, "Real Time Machine Vision System in Micromanipulator Control," presented at International Conference on Machine Automation, Osaka, Japan, 2000.
    Google ScholarLocate open access versionFindings
  • [40] I. Pappas and A. Codourey, "3D Visual Control of Microrobots," presented at Workshop on MicroMechatronics and Micro Robotics, IEEE International Conference on Robotics and Automation, Leuven, Belgium, 1998.
    Google ScholarLocate open access versionFindings
  • [41] A. Sulzmann, "3D Sensor based Microassembly and Quality Control Overview and Examples," presented at Workshop on Precision Manipulation at Micro and Nano Scales, IEEE International Conference on Robotics and Automation, Leuven, Belgium, 1998.
    Google ScholarLocate open access versionFindings
  • [42] X. Li, G. Zong, and S. Bi, "Development of Global Vision System for Biological Automatic Micro-Manipulation System," presented at IEEE International Conference on Robotics & Automation, Seoul, Korea, 2001.
    Google ScholarFindings
  • [43] Y. Mezouar and P. K. Allen, "Visual Servoed Micropositioning for Protein Manipulation Tasks.," presented at IEEE/RSJ International Conference on Intelligent Robots and Systems, Lausanne, Switzerland, 2002.
    Google ScholarLocate open access versionFindings
  • [44] H. Yamamoto and J. Sakiyama, "Stereoscopic Visual Servo System for Microinjec-tion," presented at IEEE Instrumentation and Measurement Technology Conference, Anchorage, USA, 2002.
    Google ScholarLocate open access versionFindings
  • [45] Y. Sun and B. J. Nelson, "Microrobotic Cell Injection," presented at IEEE International Conference on Robotics and Automation, Seoul, Korea, 2001.
    Google ScholarFindings
  • [46] F. A. Arámbula Cosío, L. Vega, A. Herrera Becerra, Prieto Meléndez, R. and, and C. G., "A Neural Network Based Workstation for Automated Cell Proliferation Analysis, presented at 23rd Annual EMBS International Conference, Istanbul, Turkey, 2001.
    Google ScholarFindings
  • [47] T. Jiang and F. Yang, "An Evolutionary Tabu Search for Cell Image Segmentation," IEEE Transactions on Systems, Man and Cybernetics Part B: Cybernetics, vol. 32, pp. 675 - 678, 2002.
    Google ScholarLocate open access versionFindings
  • [48] F. J. Sanchez-Marin, "Computerized Recognition of Biological Objects Using the Hotelling Transform," presented at 22nd Annual EMBS Internation Conference, Chicago, USA, 2000.
    Google ScholarLocate open access versionFindings
  • [49] S. Schüpp, A. Elmoataz, J. Fadili, P. Herlin, and D. Bloyet, "Image Segmentation via Multiple Active Contour Models and Fuzzy Clustering with Biomedical Appli-cations," presented at 15th International Conference on Pattern Recognition, 2000.
    Google ScholarFindings
  • [50] C. Zimmer, E. Labruyére, V. Meas-Yedid, N. Guillén, and J. C. Olivo-Marin, "Improving Active Contours for Segmentation and Tracking of Motile Cells in Vid-eomicroscopy.," 16th International Conference on Pattern Recognition, 2002.
    Google ScholarLocate open access versionFindings
  • [51] K. Kaneko, H. Tokashiki, K. Tanie, and K. Komoriya, "Impedance Shaping based on Force Feedback Bilateral Control in Macro-Micro Teleoperation System," presented at IEEE International Conference on Robotics and Automation, Albuquerque, New Mexico, USA, 1997.
    Google ScholarLocate open access versionFindings
  • [52] K. Park, W. K. Chung, and Y. Youm, "Obtaining Passivity of Micro-Teleoperation Handling a Small Inertia Object," presented at IEEE International Conference on Robotics & Automation, Washington DC, USA, 2002.
    Google ScholarFindings
  • [53] Y. Yokokohji, N. Hosotani, and T. Yoshikawa, "Analysis of maneverability and stability of micro-teleoperation systems," presented at IEEE International Conference on Robotics and Automation, San Diego, USA, 1994.
    Google ScholarLocate open access versionFindings
  • [54] K. Takeo and K. Kosuge, "Implementation of the Micro-macro Teleoperation System without Using Slave-side Force Sensors," presented at IEEE International Conference on Robotics and Automation, Albuquerque, New Mexico, USA, 1997.
    Google ScholarLocate open access versionFindings
  • [55] N. Ando, P. Korondi, and H. Hashimoto, "Development of Micromanipulator and Haptic Interface for Networkes Micromanipulation," IEEE/ASME Transactions on Mechatronics, vol. 6, pp. 417 - 427, 2001.
    Google ScholarLocate open access versionFindings
  • [56] D. H. Kim, B. Kim, S. Yun, and S. Kwon, "Cellular Force Measurement for Force Reflected Biomanipulation," International Conference on Robotics & Automation, pp. 2412-2417, 2004.
    Google ScholarLocate open access versionFindings
  • [57] D. H. Kim, S. Yun, and B. Kim, "Mechanical Microrobotic System," International Conference on Robotics & Automation, pp. 5013-5018, 2004.
    Google ScholarLocate open access versionFindings
  • [58] Y. Sun, K. T. Wan, K. P. Roberts, J. C. Bischof, and B. J. Nelson, "Mechanical Property Transactions on Nanobioscience, vol. 2, pp. 279-
    Google ScholarLocate open access versionFindings
  • [59] M. S. Inc, "Piezo film sensors technical manual, Internet Version, 1999.
    Google ScholarFindings
  • Mechatronics, pp. 695-700, 2001.
    Google ScholarFindings
  • [62] T. Fukuda and F. Arai, "Prototyping Design and Francisco, USA, 2000.
    Google ScholarFindings
  • [63] G. Lin, R. E. Palmer, K. S. J. Pister, and K. P. IEEE Transactions on Biomedical Engineering, vol. 48, pp. 996 - 1006, 2001.
    Google ScholarLocate open access versionFindings
  • [64] Y. Shen, N. Xi, W. J. Li, and J. Tan, "A High Mechatronics, pp. 703-708, 2003.
    Google ScholarLocate open access versionFindings
0
您的评分 :

暂无评分

标签
评论
数据免责声明
页面数据均来自互联网公开来源、合作出版商和通过AI技术自动分析结果,我们不对页面数据的有效性、准确性、正确性、可靠性、完整性和及时性做出任何承诺和保证。若有疑问,可以通过电子邮件方式联系我们:report@aminer.cn