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The average force values obtained for puncturing the outer membrane of flying fish egg cells and salmon fish egg cells was 1.6057mN and 2.2694mN respectively

Force Feedback Interface for Cell Injection

WHC, pp.391-400, (2005)

引用：57|浏览35

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dblp.uni-trier.de dl.acm.org academic.microsoft.com

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intracytoplasmic sperm injectionforce feedback interfacebiological cell injectioncell injection forceforce feedback更多(11+)

摘要

Manual pronuclei injection and intracytoplasmic sperm injection (ICSI) requires long training and has low success rates primarily due to poor control over the injection force. Consequently, there is a need for quantification of forces during biological cell injection and for an automated cell injection system, which can provide force feed...更多

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简介

Bio-surgery on individual cells, ordered cell arrays or aggregates of cells will be the platform for the generation of genetic manipulation.
To achieve bio-surgery reliably and accurately, operators must have accurate haptic (“feel”) and visual feedback from the cell as intracellular injection is conducted.
A haptic and visual feedback system can be used to manipulate an individual cell or array of cells to standardize outcomes of cellular.
Surgical procedures
Further advancement of this technology can be reliable and accurate gene injection at specific target sites within the cell or in the nucleus

重点内容

Bio-surgery on individual cells, ordered cell arrays or aggregates of cells will be the platform for the generation of genetic manipulation
This paper describes a force feedback interface for reflecting forces to the user during cell membrane puncturing tasks
The successful implementation and calibration of the force sensor has been presented in detail
The average force values obtained for puncturing the outer membrane of flying fish egg cells and salmon fish egg cells was 1.6057mN and 2.2694mN respectively
During all membrane puncture tasks the user was clearly able to discern when the membrane was punctured through a rapid drop in the force felt through the PHANToM

方法

PVDF (Polyvinylidene fluoride) piezoelectric polymer film is used to develop the force sensor for measuring the cell injection forces.
The glass micropipette is integrated to the PVDF film (Thickness: 28μm, Model: LDT1-028K of MSI, Inc.) with the help of a connector as shown in the Figure 2.
This setup allows the easy removal and replacement of the micropipette if the tip of it gets damaged during micromanipulation (5μm ID).
The following parameters will be used in the analysis: W: width of PVDF Film h: thickness of PVDF film L: length of PVDF Film A: surface Area (L * W) a: cross-sectional area (W * h) Q(t) : charge produced (PVDF Film) I(t): Current produced (PVDF film) V(t) : voltage across the PVDF Film RP : resistance of PVDF Film CP : Capacitance of the PVDF Film F(t) : Contact force

结果

To test the force feedback interface for cell injection, the authors performed several cell injections on two different types of egg cells, namely, salmon fish egg cell and flying fish egg cell.
The experimental setup consisted of the PVDF film, nanomanipulator, micropipette (Tip ID: 5μm) inserted into the hollow connector, and the PHANToM haptic interface device.
A plastic micropipette holds the

结论

This paper describes a force feedback interface for reflecting forces to the user during cell membrane puncturing tasks.
The average force values obtained for puncturing the outer membrane of flying fish egg cells and salmon fish egg cells was 1.6057mN and 2.2694mN respectively.
During all membrane puncture tasks the user was clearly able to discern when the membrane was punctured through a rapid drop in the force felt through the PHANToM.
The work presented in this paper cannot be directly applied to puncture smaller cells in the range of 50-100μm diameter.
Human factors studies comparing automated vs. manual cell injection to improve the cell viability after injection will be the natural future direction of this research

基金

* We acknowledge the support of National Science Foundation grant: CAREER IIS-

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