INHIBITION OF BIOFILM FORMATION OF BACILLUS PUMILUS1 BY ANTIMICROBIAL SUBSTANCES PRODUCED BY DIFFERENT OIL RESERVOIR BACILLUS STRAINS
msra
摘要
Biofilms are structured communities of cells encased in a polymeric matrix and adherent to a surface, interface or to each other. Microbial colonization of surfaces of petroleum industrial systems takes place through the formation of such biofilms. Problems due to biocorrosion and biofouling of industrial systems range from heavy microbiological contamination with consequent energy and efficiency losses to structural failures owing to corrosion. We report here that oil reservoir strains of Bacillus pumilus LF4, Bacillus licheniformis T6-5 and Bacillus fimus H2O-1 readily attached and grew on cover glass slide surface, forming mature biofilms of >106 cells in 6 h on a surface inoculated with the bacteria. Different antimicrobial substances (AMS) producing Bacillus species have been related to biofilm formation in the oil industry. It seems reasonable to develop strategies to avoid the colonization of disadvantageous bacteria or even the biofilm formation rather than using high doses of biocides to inhibit them after they are established. In a previous study we showed that two AMS produced by Bacillus licheniformis T6- 5 and Bacillus fimus H2O-1 were able to inhibit plancktonic sulfate reducing bacteria growth in vitro. In the present study, these same AMS were also able to eliminate Bacillus pumilus LF4 pre- established 6h-biofilm after 18h of contact with such substances. Our results suggest that the AMS produced by Bacillus licheniformis and Bacillus fimus strains may have a potential application on cleaning technologies of pipelines, preventing biofilm formation and consequently biocorrosion.
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关键词
antimicrobial substance and bacillus.,biofilm
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