Dosisabhängige Auswirkungen des kombinierten β/γ Emitters Rhenium186 auf das Wachstum humaner Gefäßwandzellen*

Purpose:The aim of this study was to evaluate the capability of human aortic smooth muse e cells (HaSMC) and endothelial cells (EC) to recover after incubation with the combined beta/gamma emitter (186)rhenium. Materials and Methods: Two days after plating, HaSMC and EC were incubated for five days with Re-186 (total doses applied 4 Gy - 32 Gy). Cell counts were performed for a period of 30 days (haSMC) and 22 days (EC). To detect possible growth recovery, colony formation assays were plated for both cell types on day 5, 10, and 20 land lay 30 for haSMC). Results: Both cell types presented a dose-dependent growth inhibition which was maximum at a dose of 32 Gy, Human endothelial cells presented with total growth recovery at 4 and 8 Cy, and a partial growth recovery at 16 Gy. Smooth muscle cells only presented partial growth recovery at 4 and 8 Cy. At 16 Gy and mo-re no recovery was detected. Conclusion: HaSMC as well as EC growth can be modulated effectively with Re-186 over a period of 30 days in vitro. Compared to smooth muscle cells human endothelial cells seem to possess a higher potential to recover at doses of 8 to 16 Gy. Re-186 may be a valuable radionuclide to prevent restenosis.