Significant Accumulation Of C-4-Specific Pyruvate,Orthophosphate Dikinase In A C-3 Plant, Rice
PLANT PHYSIOLOGY(2001)
摘要
The C-4-Pdk gene encoding the C-4 enzyme pyruvate, orthophosphate dikinase (PPDK) of maize (Zea mays cv Golden Cross Bantam) was introduced into the C-3 plant, rice (Oryza sativa cv Kitaake). When the intact maize C-4-Pdk gone, containing its own promoter and terminator sequences and exon/intron structure, was introduced, the PPDK activity in the leaves of some transgenic lines was greatly increased, in one line reaching 40-fold over that of wild-type plants. In a homozygous line, the PPDK protein accounted for 35% of total leaf-soluble protein or 160% of total leaf nitrogen. In contrast, introduction of a chimeric gene containing the full-length cDNA of the maize PPDK fused to the maize C-4-Pdk promoter or the rice Cab promoter only increased PPDK activity and protein level slightly. These observations suggest that the intron(s) or the terminator sequence of the maize gene, or a combination of both, is necessary for high-level expression. In maize and transgenic rice plants carrying the intact maize gene, the level of transcript in the leaves per copy of the maize C-4-Pdk gone was comparable, and the maize gene was expressed in a similar organ-specific manner. These results suggest that the maize C-4-Pdk gene behaves in a quantitatively and qualitatively similar way in maize and transgenic rice plants. The activity of the maize PPDK protein expressed in rice leaves was light/dark regulated as it is in maize. This is the first reported evidence for the presence of an endogenous PPDK regulatory protein in a C-3 plant.
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关键词
wild type,enzyme,transgenic rice,protein expression
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