Transcriptional analysis of intracytoplasmically stained, FACS-purified cells by high-throughput, quantitative nuclease protection
NATURE BIOTECHNOLOGY(2009)
摘要
Cells that have been purified by FACS using intracellular markers are not amenable to gene expression analysis by conventional methods. Pechhold et al . solve this problem with the quantitative nuclease protection assay and apply the approach to study subsets of islet cells.
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关键词
nature,biotechnology,high throughput
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