Dna Breakage Induced By 1,2,4-Benzenetriol: Relative Contributions Of Oxygen-Derived Active Species And Transition Metal Ions

We report here the relative roles of metals and selected reactive oxygen species in DNA damage by the genotoxic benzene metabolite 1,2,3-benzenetriol, and the interactions of antioxidants in affording protection. 1,2,4-Benzenetriol induces scission in supercoiled phage DNA in neutral aqueous solution with an effective dose (ED50) of 6.7 muM for 50% cleavage of 2.05 mug/ml supercoiled PM2 DNA. in decreasing order of effectiveness: catalase (20 U/ml), formate (25 mM), superoxide dismutase (20 U/ml), and mannitol (50 mM) protected, from 85 to 28%. Evidently, H2O2 is the dominant active species, with O-2(.-) and (OH)-O-. playing subordinate roles. Desferrioxamine or EDTA inhibited DNA breakage by 81-85%, despite accelerating 1,2,3-benzenetriol autoxidation. Consistent with this suggestion of a crucial role For metals, addition of cupric, cuprous, ferric, or ferrous ions enhanced DNA breakage, with copper being more active than iron. Combinations of scavengers protected more effectively than any single scavenger alone, with implications for antioxidants acting in concert in living cells. Synergistic combinations were superoxide dismutase with (OH)-O-. scavengers, superoxide dismutase with desferrioxamine, and catalase with desferrioxamine, Antagonistic (preemptive) combinations were catalase with superoxide dismutase, desferrioxamine with (OH)-O-. scavengers, and catalase with (OH)-O-. scavengers. The most striking aspect of synergism was the extent to which metal chelation (desferrioxamine) acted synergistically with either catalase or superoxide dismutase to provide virtually complete protection. Concluding, 1,2,4-benzenetriol-induced DNA damage occurs mainly by site-specific, Fenton-type mechanisms, involving synergism between several reactive intermediates. Multiple antioxidant actions are needed for effective protection. (C) 2001 Elsevier Science Inc.