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In this work we have investigated extracellular biosynthesis of silver nanoparticles using Aspergillus fumigatus

Extracellular biosynthesis of silver nanoparticles using the fungus Aspergillus fumigatus

Colloids and Surfaces B: Biointerfaces, no. 2 (2006): 160-164

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摘要

Development of reliable and eco-friendly process for synthesis of metallic nanoparticles is an important step in the filed of application of nanotechnology. One of the options to achieve this objective is to use natural processes such as use of biological systems. In this work we have investigated extracellular biosynthesis of silver nano...更多

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简介
  • Increased industrialization and urbanization has damaged the environment by introducing a number of harmful and unwanted substances.
  • The mechanisms include: efflux systems; alteration of solubility and toxicity via reduction or oxidation; biosorption; bioaccumulation; extracellular complexation or precipitation of metals and lack of specific metal transport systems [1,2].
  • These metal–microbe interactions have important role in several biotechnological applications including the fields of bioremediation, biomineralization, bioleaching and microbial corrosion.
  • It is only recently that microorganisms have been explored as potential biofactory for synthesis of metal-
重点内容
  • Increased industrialization and urbanization has damaged the environment by introducing a number of harmful and unwanted substances
  • Application of silver nanoparticles in these fields is dependent on the ability to synthesize particles with different chemical composition, shape, size, and monodispersity
  • There are several physical and chemical methods for synthesis of metallic nanoparticles that are followed by the material scientists currently [11]
  • Nature has provided exciting possibilities of utilizing biological systems for this purpose. This comes from the fact that microorganisms while interacting with metal ions have shown to reduce the ions into metallic particles
  • Both bacteria and fungi have shown ability to reduce metal ions to form metallic nanoparticles. It would be advantageous if a fungus is used for the development of a process keeping in mind handling of the biomass and down stream processing of the nanoparticles [3]. In this regard extracellular biosynthesis of silver nanoparticles achieved in this study using A. fumigatus may prove to be an important step in the right direction
方法
  • A. fumigatus (NCIM 902) was obtained from National Chemical Laboratory, Pune, India and maintained on potato dextrose agar slants.
  • The flasks were inoculated, incubated on orbital shaker at 25 ◦C and agitated at 150 rpm.
  • 20 g of biomass was brought in contact with 200 ml of Milli-Q deionized water for 72 h at 25 ◦C in an Erlenmeyer flask and agitated in the same condition as described earlier.
  • The cell filtrate was obtained by passing it through Whatman filter paper no
结果
  • The detailed study on extracellular biosynthesis of silver nanoparticles by the Aspergillus biomass was carried out in this work.
  • The flasks being incubated in the dark in an environmental shaker showed gradual change in color of the medium to brown, with intensity increasing during the period of incubation.
  • Control showed no change in color of the cell filtrate when incubated in the same environmental condition
结论
  • Silver nanoparticles have many important applications that include: spectrally selective coating for solar energy absorption and intercalation material for electrical batteries [5], as optical receptors [8], polarizing filters, catalysts in chemical reaction, biolabelling [9] and as antimicrobial agents [10].
  • Development of simple and eco-friendly synthetic route would help promoting further interest in the synthesis and application of metallic nanoparticles
  • In this respect, nature has provided exciting possibilities of utilizing biological systems for this purpose.
  • Both bacteria and fungi have shown ability to reduce metal ions to form metallic nanoparticles
  • It would be advantageous if a fungus is used for the development of a process keeping in mind handling of the biomass and down stream processing of the nanoparticles [3].
  • In this regard extracellular biosynthesis of silver nanoparticles achieved in this study using A. fumigatus may prove to be an important step in the right direction
总结
  • Introduction:

    Increased industrialization and urbanization has damaged the environment by introducing a number of harmful and unwanted substances.
  • The mechanisms include: efflux systems; alteration of solubility and toxicity via reduction or oxidation; biosorption; bioaccumulation; extracellular complexation or precipitation of metals and lack of specific metal transport systems [1,2].
  • These metal–microbe interactions have important role in several biotechnological applications including the fields of bioremediation, biomineralization, bioleaching and microbial corrosion.
  • It is only recently that microorganisms have been explored as potential biofactory for synthesis of metal-
  • Methods:

    A. fumigatus (NCIM 902) was obtained from National Chemical Laboratory, Pune, India and maintained on potato dextrose agar slants.
  • The flasks were inoculated, incubated on orbital shaker at 25 ◦C and agitated at 150 rpm.
  • 20 g of biomass was brought in contact with 200 ml of Milli-Q deionized water for 72 h at 25 ◦C in an Erlenmeyer flask and agitated in the same condition as described earlier.
  • The cell filtrate was obtained by passing it through Whatman filter paper no
  • Results:

    The detailed study on extracellular biosynthesis of silver nanoparticles by the Aspergillus biomass was carried out in this work.
  • The flasks being incubated in the dark in an environmental shaker showed gradual change in color of the medium to brown, with intensity increasing during the period of incubation.
  • Control showed no change in color of the cell filtrate when incubated in the same environmental condition
  • Conclusion:

    Silver nanoparticles have many important applications that include: spectrally selective coating for solar energy absorption and intercalation material for electrical batteries [5], as optical receptors [8], polarizing filters, catalysts in chemical reaction, biolabelling [9] and as antimicrobial agents [10].
  • Development of simple and eco-friendly synthetic route would help promoting further interest in the synthesis and application of metallic nanoparticles
  • In this respect, nature has provided exciting possibilities of utilizing biological systems for this purpose.
  • Both bacteria and fungi have shown ability to reduce metal ions to form metallic nanoparticles
  • It would be advantageous if a fungus is used for the development of a process keeping in mind handling of the biomass and down stream processing of the nanoparticles [3].
  • In this regard extracellular biosynthesis of silver nanoparticles achieved in this study using A. fumigatus may prove to be an important step in the right direction
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