Protective Effect of Jianpi Yangrong Tablets on Mice with Cyclophosphamide-induced Low Immunity

Objective To observe the effect of Jianpi Yangrong Tablets (JYT) on mice with cyclophosphamide-induced low immunity. Methods Sixty NIH mice were equally randomized into 6 groups: low-, middle-and high-dose JYT groups (4、8 and 16gkg-1d-1 respectively), batyl alcohol group (0.055gkg-1d-1), model group and normal group. The mice received gastric gavage of the corresponding drug according to the experimental design. On the 5th day of medication, the mice received intraperitoneal injection of cyclophosphamide 200mg/kg to induce the models of low immunity. On the 9th day of medication, peripheral white blood cell (WBC) count, carbon clearance test, bone marrow nucleated cells count, spleen index and proliferative reaction of spleen lymphocyteswere observed.Results The number of WBC in middle-and high-dose JYT groups and batyl alcohol group was higher than that in the model group (P0.01), and the number of WBC in the middle-dose JYT group was higher that that in batyl alcohol group (P0.05). The carbon clearance index K was higher in middle-and high-dose JYT groups than that in the model group (P0.05), and after correction α value of carbon clearance index in the middle-dose JYT group was higher (P0.01). The number of bone marrow nucleated cells in middle-dose JYT groups was higher than that in the model group (P0.01),low-dose JYT group and batyl alcohol group (P0.05). Compared with the model group, spleen index was increased in the middle-dose JYT group and batyl alcohol group (P0.05), and the inhibition of cyclophosphamide on proliferative reaction of spleen lymphocytes was counteracted by the middle-and high-dose JYT and batyl alcohol (P0.01).Conclusion JYT can enhance the immune function of mice with low immunity and increase the peripheral WBC count in mice.