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We report on a feasible method to construct a nanoparticle-enzyme biosensor

Covalent attachment of glucose oxidase to an Au electrode modified with gold nanoparticles for use as glucose biosensor.

Bioelectrochemistry, no. 1 (2005): 15-22

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摘要

A feasible method to fabricate glucose biosensor was developed by covalent attachment of glucose oxidase (GOx) to a gold nanoparticle monolayer modified Au electrode. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) of ferrocyanide followed and confirmed the assemble process of biosensor, and indicated that the gol...更多

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简介
  • In the last few years, considerable attention has been devoted to immobilization of biomolecules, especially enzymes on electrode surface in relation to the development of biosensors and biotechnological processes.
  • A cystamine monolayer was chemisorbed onto those gold nanoparticles and exposed an array of amino groups, which would further react with aldehyde groups of periodate oxidized GOx via the wellknown Schiff base reaction.
重点内容
  • In the last few years, considerable attention has been devoted to immobilization of biomolecules, especially enzymes on electrode surface in relation to the development of biosensors and biotechnological processes
  • The combination of nanometer materials and biomolecules is of considerable interest in the fields of biotechnology and bioanalytical chemistry, because nanoparticles can play an important role in improving the biosensor performance due to their large specific surface area and excellent biocompatibility
  • On the basis of cyclic voltammetry and electrochemical impedance investigations, we demonstrated that glucose oxidase (GOx) molecules are successfully and stably immobilized onto Au electrode, and the self-assembled gold nanoparticles have a marked influence on the interface property of the modified electrode and play an important role in improving the analytical performance of biosensor
  • Scheme 1 shows the stepwise fabrication process of biosensor, which was followed by cyclic voltammetry using a ferrocyanide as redox probe
  • Cyclic voltammetry of ferrocyanide is a valuable and convenient tool to monitor the barrier of the modified electrode
  • The proposed construction method is of interest for the development of glucose biosensors, especially miniaturized biosensors that have minimum electrode area
结果
  • Scheme 1 shows the stepwise fabrication process of biosensor, which was followed by cyclic voltammetry using a ferrocyanide as redox probe.
  • Cyclic voltammetry of ferrocyanide is a valuable and convenient tool to monitor the barrier of the modified electrode.
  • SH SH SH gold nanoparticles S S cystamine O HC H O= C GOx C= O
结论
  • The authors have developed a novel way to fabricate glucose biosensor by covalent attachment of glucose oxidase to a gold nanoparticle monolayer modified Au electrode.
  • The resulting biosensor exhibits many advantages, such as high sensitivity, low detection limit, higher affinity for glucose, good reproducibility and storage stability, and so on.
  • The improved electrode coverage and high sensitivity are important steps towards further miniaturization of biosensors.
  • The proposed construction method is of interest for the development of glucose biosensors, especially miniaturized biosensors that have minimum electrode area
总结
  • Introduction:

    In the last few years, considerable attention has been devoted to immobilization of biomolecules, especially enzymes on electrode surface in relation to the development of biosensors and biotechnological processes.
  • A cystamine monolayer was chemisorbed onto those gold nanoparticles and exposed an array of amino groups, which would further react with aldehyde groups of periodate oxidized GOx via the wellknown Schiff base reaction.
  • Results:

    Scheme 1 shows the stepwise fabrication process of biosensor, which was followed by cyclic voltammetry using a ferrocyanide as redox probe.
  • Cyclic voltammetry of ferrocyanide is a valuable and convenient tool to monitor the barrier of the modified electrode.
  • SH SH SH gold nanoparticles S S cystamine O HC H O= C GOx C= O
  • Conclusion:

    The authors have developed a novel way to fabricate glucose biosensor by covalent attachment of glucose oxidase to a gold nanoparticle monolayer modified Au electrode.
  • The resulting biosensor exhibits many advantages, such as high sensitivity, low detection limit, higher affinity for glucose, good reproducibility and storage stability, and so on.
  • The improved electrode coverage and high sensitivity are important steps towards further miniaturization of biosensors.
  • The proposed construction method is of interest for the development of glucose biosensors, especially miniaturized biosensors that have minimum electrode area
基金
  • This work was supported by the Major State Basic Research Development Program (G2000078102), Educational Ministry (20020183007), National Natural Science Foundation (20175007) of China, and Key Lab of Supramolecular Structure and Materials of Ministry of Education, Jilin University
研究对象与分析
data: 10
Ip was measured from the background-corrected current value by subtracting the glucose-free current to glucosecontain current at a potential of 0.3 V. The average Ip obtained from 10 different electrodes (n=10) was (3.6F0.1). was estimate to be

data: 10
For comparison, when GOx was directly attached to cystamine modified gold electrode (marked as. Au/cystamine/GOx), only a smaller catalytic current was observed as shown in cystamine/GOx electrode is (0.9F0.1)Â10À12 mold cmÀ2 (n=10), less than one fourth of that obtained at Au/dithiol/. au/cystamine/GOx electrode

data: 10
Fig. 6 shows a calibration plot of the steady-state current vs. glucose concentration. The current response of the sensor is linear in the range between 2.0Â10À5 M and 5.7Â10À3 M with sensitivity of (8.8F0.2) AAd mMÀ1d cmÀ2 (n=10). The detection limit of the sensor was determined to be 8.2 AM at a signal to noise ratio of 3

data: 10
The reproducibility of the biosensors was examined in at 20 mM glucose solution (0.1 mM ferrocenemethanol as mediator). The relative standard deviation was 2.1% (n=10). The electrode-to-electrode reproducibility was also examined between ten different electrodes in above solutions, and the relative standard deviation was calculated to be 4.2%

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